Role of trapped air in the formation of cell-and-protein micropatterns on superhydrophobic/superhydrophilic microtemplated surfaces

CORC > 厦门大学 > 化学化工－已发表论文

	Role of trapped air in the formation of cell-and-protein micropatterns on superhydrophobic/superhydrophilic microtemplated surfaces
	Huang, Qiaoli ; Lin, Longxiang ; Yang, Yun ; Hu, Ren ; Vogler, Erwin ; Lin, Changjian ; Hu R(胡仁) ; Lin CJ(林昌健)
刊名	http://dx.doi.org/10.1016/j.biomaterials.2012.08.017
	2012
关键词	Biochemistry Cell adhesion Cells Hydrophobicity Mammals Proteins
英文摘要	Air trapped within the interstices of TiO2 nanotube surfaces bearing superhydrophobic/superhydrophilic microtemplated domains controls formation of protein micropatterns but not cell micropatterns. Protein binding from either bovine-serum albumin (BSA) or fetal-bovine serum (FBS) solutions to superhydrophobic domains is blocked in the presence of trapped air, leading to clear protein binding contrast between superhydrophilic and superhydrophobic domains. Protein binds to superhydrophobic domains when air is displaced by sonication, leading to more protein binding to superhydrophobic domains than to superhydrophilic, with concomitantly blurred protein binding contrast. The overall contrast obtained in formation of cell (hFOB1.19, MG63, and HeLa) micropatterns depends on the cell type and protein composition of the fluid phase. All cell types preferentially attach to superhydrophilic domains from each fluid phase tested (FBS, BSA, and basal media containing no protein). All cell types do not attach to superhydrophobic domains from FBS solutions, with-or-without trapped air, creating a visually-obvious cell attachment pattern. However, cells attached to superhydrophobic domains from basal media suspensions, with-or-without trapped air, creating a blurred cell attachment pattern. Cell attachment from BSA-containing solutions gave mixed results depending on cell type. Thus, trapped air does not necessarily block cell attachment as has been suggested in the literature. Rather, cell attachment is controlled by interfacial tensions between cells, surfaces, and fluid phases in a manner that can be understood in terms of the Dupre麓 work-of-adhesion formulation. Cell attachment patterns developed within the initial attachment phase persist for up to two days of continuous culture but overgrow thereafter, with-or-without trapped air, showing that trapped air does not block cell overgrowth over time of continuous culture. 漏 2012 Elsevier Ltd.
语种	英语
出版者	Elsevier Ltd
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/89586]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Huang, Qiaoli,Lin, Longxiang,Yang, Yun,et al. Role of trapped air in the formation of cell-and-protein micropatterns on superhydrophobic/superhydrophilic microtemplated surfaces[J]. http://dx.doi.org/10.1016/j.biomaterials.2012.08.017,2012.
APA	Huang, Qiaoli.,Lin, Longxiang.,Yang, Yun.,Hu, Ren.,Vogler, Erwin.,...&林昌健.(2012).Role of trapped air in the formation of cell-and-protein micropatterns on superhydrophobic/superhydrophilic microtemplated surfaces.http://dx.doi.org/10.1016/j.biomaterials.2012.08.017.
MLA	Huang, Qiaoli,et al."Role of trapped air in the formation of cell-and-protein micropatterns on superhydrophobic/superhydrophilic microtemplated surfaces".http://dx.doi.org/10.1016/j.biomaterials.2012.08.017 (2012).