A novel signal-on DNAzyme-based electrochemiluminescence, sensor for Pb2+

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	A novel signal-on DNAzyme-based electrochemiluminescence, sensor for Pb2+
	Wu, Yan-fang ; Cai, Zhi-min ; Wu, Geng-huang ; Rong, Ming-cong ; Jiang, Ya-qi ; Yang, Chao-yong James ; Chen, Xi ; Jiang YQ(蒋亚琪) ; Yang CY(杨朝勇) ; Chen X(陈曦)
刊名	http://dx.doi.org/10.1016/j.snb.2013.09.050
	2014
关键词	ACID) MODIFIED ELECTRODE QUANTUM DOTS LEAD CHEMILUMINESCENCE BIOSENSOR SURFACE
英文摘要	National Nature Scientific Foundation of China [20975085, 21175112]; National Basic Research Program of China [2010CB732402]; A novel signal-on electrochemiluminescence (ECL) biosensor for the determination of lead (Pb2+) was developed. Background suppression for the ECL detection of Pb2+ and hybridization in the presence of Pb2+ were realized using a hairpin probe. The 8-17 DNAzymes, which hybridized with their substrate strands, were immobilized onto the surface of a glassy carbon electrode (GCE) via amino coupling method. In the presence of Pb2+, the substrate strands were cleaved and released, while DNAzymes alone remained on the GCE surface. The single-stranded DNAzyme could hybridize with a hairpin probe containing an ECL luminophore resulting in quantitative ECL signals. The sequence of the hairpin probe was designed and optimized for hybridizing with single-stranded DNAzyme but not with DNAzyme-substrate double strands. This signal-on strategy possessed low ECL background signals, high sensitivity and selectivity toward Pb2+. The ECL sensing approach presented excellent response to Pb2+ from 10 pM to 100 nM with a detection limit of 6.4 pM. (C) 2013 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE SA
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/89406]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Wu, Yan-fang,Cai, Zhi-min,Wu, Geng-huang,et al. A novel signal-on DNAzyme-based electrochemiluminescence, sensor for Pb2+[J]. http://dx.doi.org/10.1016/j.snb.2013.09.050,2014.
APA	Wu, Yan-fang.,Cai, Zhi-min.,Wu, Geng-huang.,Rong, Ming-cong.,Jiang, Ya-qi.,...&陈曦.(2014).A novel signal-on DNAzyme-based electrochemiluminescence, sensor for Pb2+.http://dx.doi.org/10.1016/j.snb.2013.09.050.
MLA	Wu, Yan-fang,et al."A novel signal-on DNAzyme-based electrochemiluminescence, sensor for Pb2+".http://dx.doi.org/10.1016/j.snb.2013.09.050 (2014).