Direct proteomic mapping of Streptomyces roseosporus NRRL 11379 with precursor and insights into daptomycin biosynthesis

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	Direct proteomic mapping of Streptomyces roseosporus NRRL 11379 with precursor and insights into daptomycin biosynthesis
	Ye, Chiming ; Ng, I-Son ; Jing, Keju ; Lu, Yinghua ; Jing KJ(敬科举) ; Lu YH(卢英华)
刊名	http://dx.doi.org/10.1016/j.jbiosc.2013.10.021
	2014
关键词	TOP-DOWN MASS-SPECTROMETRY BOTTOM-UP COELICOLOR OPTIMIZATION VANCOMYCIN GROWTH RNA
英文摘要	Fundamental Research Funds for the Central Universities [2011121017]; Fujian Provincial Department of Science Technology [2012I0009]; Chinese National Natural Science Foundation [21206141]; This first-attempt study provided liquid chromatography tandem mass (LC-MS/MS) proteomics approach to explore precursor effects on daptomycin synthesis from Streptomyces roseosporus NRRL 11379. Among all, 357 and 691 differential proteins from 601 proteins in precursor group (144 h+) and 935 proteins in non-precursor group (144 h-) were identified, respectively. Through the simulation of the 2D-protein mapping, most proteins were found in isoelectric points ranged of 4.5-10.0 as well as Mws ranged 10-100 kDa. As a result, LC-MS/MS analysis was consistence with the analytical results of two-dimensional electrophoresis (2DE) but provided much intact profiles of proteins by precursor effect on S. roseosporus. To have more insight exploration, differential proteins associated to Streptomyces spp. were defined into 14 groups of their functional classification. The major differential proteins were in transport/membrane functional group with an occupation of 12.4% for 144 h+ and 5.2% for 144 h-, respectively. LC-MS/MS results as a direct proteomic mapping approach reveal more daptomycin synthetic and regulation-related proteins from precursor group in terms of methyltransferase, ATP-binding cassette (ABC) transporters, resistance proteins and regulators. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.
语种	英语
出版者	SOC BIOSCIENCE BIOENGINEERING JAPAN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/89305]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Ye, Chiming,Ng, I-Son,Jing, Keju,et al. Direct proteomic mapping of Streptomyces roseosporus NRRL 11379 with precursor and insights into daptomycin biosynthesis[J]. http://dx.doi.org/10.1016/j.jbiosc.2013.10.021,2014.
APA	Ye, Chiming,Ng, I-Son,Jing, Keju,Lu, Yinghua,敬科举,&卢英华.(2014).Direct proteomic mapping of Streptomyces roseosporus NRRL 11379 with precursor and insights into daptomycin biosynthesis.http://dx.doi.org/10.1016/j.jbiosc.2013.10.021.
MLA	Ye, Chiming,et al."Direct proteomic mapping of Streptomyces roseosporus NRRL 11379 with precursor and insights into daptomycin biosynthesis".http://dx.doi.org/10.1016/j.jbiosc.2013.10.021 (2014).