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	A long-lifetime Ru(II) metal-ligand complex as a membrane probe
	Guo, X. Q. ; Castellano, F. N. ; Li, L. ; Lakowicz, J. R. ; Guo XQ(郭祥群)
	1998
关键词	EXCITED-STATE INTERACTIONS DIPYRIDOPHENAZINE COMPLEXES LIGHT SWITCH DNA RUTHENIUM(II) 1,6-DIPHENYL-1,3,5-HEXATRIENE VESICLES
英文摘要	A luminescent metal-ligand complex, [Ru(bpy)(2)(dppz)](2+), (where dppz is dipyrido[3,2-a:2',3'-c] phenazine), was used as a photoluminescence probe for investigating submicrosecond lipid dynamics in a dipalmitoyl-L-alpha-phosphotidylglycerol (DPPG) model bilayer system. The luminescence of [Ru(bpy)(2)(dppz)](2+) in buffer is completely quenched but becomes luminescent when intercalated into DPPG vesicles. The experimental results show that the emission intensity of [Ru(bpy)(2)(dppz)](2+) intercalated into DPPG vesicles increases dramatically as temperature is increased towards the lipid phase transition temperature. This effect is abolished in bilayers containing a high concentration (> 30 mol%) of cholesterol, suggesting this probe is sensitive to the membrane composition. Frequency-domain emission intensity decays, measured as a function of increasing temperature towards the lipid phase transition temperature (2 to 57 degrees C), display two major lifetime components. The short lifetime disappears at temperatures well above the phase transition temperature. A comparison of oxygen quenching with iodide quenching suggests the heterogeneity of probe location at temperatures well below the lipid phase transition temperature and the homogeneity of probe location at temperatures well above the lipid phase transition temperature. [Ru(bpy)(2)(dppz)](2+) displays polarized emission, enabling the study of membrane dynamics. The long decay time displayed by this probe allows measurement of the overall rotational correlation time of lipid vesicles on the microsecond time-scale. Because of the long lifetime, polarized emission, and background free nature of the photoluminescence measurements, [Ru(bpy)(2)(dppz)](2+) has numerous applications in the biophysical studies of membranes. (C) 1998 Elsevier Science B.V.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/64060]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Guo, X. Q.,Castellano, F. N.,Li, L.,et al. A long-lifetime Ru(II) metal-ligand complex as a membrane probe[J],1998.
APA	Guo, X. Q.,Castellano, F. N.,Li, L.,Lakowicz, J. R.,&郭祥群.(1998).A long-lifetime Ru(II) metal-ligand complex as a membrane probe..
MLA	Guo, X. Q.,et al."A long-lifetime Ru(II) metal-ligand complex as a membrane probe".(1998).

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