Characterization of the oligomerization and ligand-binding properties of recombinant rat lipocalin 11

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	Characterization of the oligomerization and ligand-binding properties of recombinant rat lipocalin 11
	Gu, Yina ; Liu, Qiang ; Chen, Peiyan ; Guo, Chenyun ; Liu, Yan ; Zhao, Yufen ; Zhang, Yonglian ; Lin, Donghai ; Zhao YF(赵玉芬)
刊名	http://dx.doi.org/10.1016/j.bbapap.2012.08.018
	2013-01
关键词	Lipocalin Lcn11 Dimer Disulfide bond Ligand-binding beta-barrel
英文摘要	Lipocalin 11 (Lcn11), a recently identified member of the lipocalin family, potentially plays crucial physiological roles in male reproduction. In this present work, we cloned, expressed and purified the rat Lcnl11 (rLcn11) protein in Escherichia coli. A C59A/C156A substitution was introduced to ameliorate the misfolding and aggregation problem associated with the wild-type protein. From circular dichroism and non-reducing SDS-PAGE, we characterized the conformational properties of rLcn11 as a typical lipocalin scaffold with the conserved disulfide bridge. The results obtained from size-exclusion chromatography, cross-linking experiment and dynamic light scattering analysis indicate that the recombinant rLcn11 protein forms dimer in neutral solution. By using fluorescent probe 8-anilino-1-naphtahlene sulfonic acid (ANS), we found rLcn11 might contain multiple hydrophobic binding sites for ligand binding. Similarly to the odorant-binding protein, rLcn11 processes a moderate affinity for binding 1-aminoanthracene (AMA), implying that Lcn11 might work as a dimeric chemoreception protein in male reproductive system. (C) 2012 Elsevier B.V. All rights reserved.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/63757]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Gu, Yina,Liu, Qiang,Chen, Peiyan,et al. Characterization of the oligomerization and ligand-binding properties of recombinant rat lipocalin 11[J]. http://dx.doi.org/10.1016/j.bbapap.2012.08.018,2013.
APA	Gu, Yina.,Liu, Qiang.,Chen, Peiyan.,Guo, Chenyun.,Liu, Yan.,...&赵玉芬.(2013).Characterization of the oligomerization and ligand-binding properties of recombinant rat lipocalin 11.http://dx.doi.org/10.1016/j.bbapap.2012.08.018.
MLA	Gu, Yina,et al."Characterization of the oligomerization and ligand-binding properties of recombinant rat lipocalin 11".http://dx.doi.org/10.1016/j.bbapap.2012.08.018 (2013).