Use of biodiesel-derived crude glycerol for vancomycin production by Amycolatopsis orientalis XMU-VS01

CORC > 厦门大学 > 化学化工－已发表论文

	Use of biodiesel-derived crude glycerol for vancomycin production by Amycolatopsis orientalis XMU-VS01
	Zeng, Xianhai ; Wang, Sili ; Jing, Keju ; Zhang, Zhixiang ; Lu, Yinghua ; Lu YH(卢英华)
刊名	http://dx.doi.org/10.1002/elsc.201200062
	2013-01
关键词	Amycolatopsis orientalis fermentation Biodiesel Crude glycerol Vancomycin
英文摘要	Crude glycerol is a primary by-product in the biodiesel industry. Microbial fermentation on crude glycerol for producing value-added products provides opportunities to utilize a large quantity of this by-product. This study investigates the potential of using the crude glycerol to produce vancomycin (glycopeptide antibiotics) through fermentation of Amycolatopsis orientalis XMU-VS01. The results show that crude glycerol was the most effective carbon source for mycelium growth and vancomycin production, with 4060 g/L glycerol concentration as optimal range. Among other culture medium components, potato protein (nitrogen source) and the phosphate concentration had significant effects (p<0.05) for vancomycin production. A Box-Behnken design and response surface methodology were employed to formulate the optimal medium. Their optimal values were determined as 52.73 g/L of glycerol, 17.36 g/L of potato protein, and 0.1 g/L of dipotassium phosphate. A highest vancomycin yield of 7.61 g/L with biomass concentration of 15.8 g/L was obtained after 120 h flask fermentation. The yield of vancomycin was 3.5 times higher than with basic medium. The results suggest that biodiesel-derived crude glycerol is a promising feedstock for production of vancomycin from A. orientalis culture.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/61362]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Zeng, Xianhai,Wang, Sili,Jing, Keju,et al. Use of biodiesel-derived crude glycerol for vancomycin production by Amycolatopsis orientalis XMU-VS01[J]. http://dx.doi.org/10.1002/elsc.201200062,2013.
APA	Zeng, Xianhai,Wang, Sili,Jing, Keju,Zhang, Zhixiang,Lu, Yinghua,&卢英华.(2013).Use of biodiesel-derived crude glycerol for vancomycin production by Amycolatopsis orientalis XMU-VS01.http://dx.doi.org/10.1002/elsc.201200062.
MLA	Zeng, Xianhai,et al."Use of biodiesel-derived crude glycerol for vancomycin production by Amycolatopsis orientalis XMU-VS01".http://dx.doi.org/10.1002/elsc.201200062 (2013).