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	One-pot preparation of basic amino acid-silica hybrid monolithic column for capillary electrochromatography
	Xu, Hongrui ; Xu, Zhendong ; Yang, Limin ; Yang LM(杨利民) ; Wang, Qiuquan ; Wang QQ(王秋泉)
	2011-05-13
关键词	Basic amino acid CEC Hybrid silica monolithic column L-Lysine-silica hybrid monolithic column Zwitterionic stationary phase
英文摘要	A novel "one-pot" strategy was developed for the preparation of amino acid (AA)-silica hybrid monolithic column. The basic AA (L-Arginine, L-Lysine and L-Histidine) was covalently incorporated into the silica hybrid skeleton via the epoxy ring-opening reaction between the amine group and the glycidyl moiety in gamma-glycidoxypropyltrimethoxysilane (GPTMS), which was confirmed by elemental analysis and FT-IR studies, while the basic AA was also found to catalyze the polycondensation of tetramethoxysilane and GPTMS. The average mesopore and macropore sizes of the prepared basic AA-silica hybrid monolithic columns were 3.86 nm and 1.71 mu m for the L-Lysine-silica hybrid monolith, 5.38 nm and 4.24 mu m for the L-Arginine-silica hybrid monolith, and 6.38 nm and 1.24 mu m for the L-Histidine-silica hybrid monolith. The hybrid monolith afforded a zwitterionic stationary phase for CEC, the direction and magnitude of EOF can be controlled by the pH of the mobile phase used. Besides an electrophoretic mechanism, the monoliths behave in a typical hydrophilic interaction with the analytes when ACN percentage in the mobile phase is over 40%. Four polar compounds (toluene, DMF, formamide and thiourea) were tested on the three AA-silica hybrid monolithic columns, and the best separation efficiency was observed in the L-Lysine-silica hybrid monolithic column, its theoretical plate height was down to 5.7 gm for thiourea when 20 mM HCOOH-HCOONH(4) containing 20% ACN (pH 4.1) was used as a running buffer. The corresponding theoretical plate number for toluene, DMF, formamide and thiourea were 123 385, 103 620, 121 845 and 105 345 plates/m, respectively. Effective separation of phenols and peptides on the L-Lysine-silica hybrid monolithic column was achieved using CEC. We believe that this strategy paves a way for the easy preparation of various functional silica hybrid monolithic columns, aiming at different separation purposes.; National Natural Science Foundation of China[21035006, 20775062]; 973 program[2009CB421605]
语种	英语
出版者	WILEY-BLACKWELL
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1002/jssc.201100177]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Xu, Hongrui,Xu, Zhendong,Yang, Limin,et al. One-pot preparation of basic amino acid-silica hybrid monolithic column for capillary electrochromatography[J],2011.
APA	Xu, Hongrui,Xu, Zhendong,Yang, Limin,杨利民,Wang, Qiuquan,&王秋泉.(2011).One-pot preparation of basic amino acid-silica hybrid monolithic column for capillary electrochromatography..
MLA	Xu, Hongrui,et al."One-pot preparation of basic amino acid-silica hybrid monolithic column for capillary electrochromatography".(2011).

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