| In vitro selection of DNA aptamer against abrin toxin and aptamer-based abrin direct detection | |
| Tang, Jijun ; Yu, Tao ; Yu T(于涛) ; Guo, Lei ; Xie, Jianwei ; Shao, Ningsheng ; He, Zhike | |
| 2006-10 | |
| 关键词 | in vitro selection aptamer abrin luminescence |
| 英文摘要 | Abrin toxin as the target protein, belongs to class II ribosome-inactivating proteins family, has high toxicity to eukaryotic cells. Here, we firstly report the DNA aptamers, isolated by in vitro selection, recognize abrin toxin with high affinity and specificity, and have the advantage of no cross-reaction with structure-similar protein ricin toxin over antibodies. Then, a highly selective and sensitive aptamer-based abrin assay was established using a molecular light switching reagent [Ru(phen)(2)(dppz)](2+) with a limit of detection of 1 nM and a wide linear range from 1 to 400 nM with the correlation coefficient of 0.993. This assay can be successfully directly performed not only in physiological buffer but also in more complicated biological matrix, such as diluted serum. (c) 2006 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 内容类型 | 期刊论文 |
| 源URL | [http://dx.doi.org/doi:10.1016/j.bios.2006.09.015]  |
| 专题 | 化学化工－已发表论文 |
| 推荐引用方式 GB/T 7714 | Tang, Jijun,Yu, Tao,Yu T,et al. In vitro selection of DNA aptamer against abrin toxin and aptamer-based abrin direct detection[J],2006. |
| APA | Tang, Jijun.,Yu, Tao.,于涛.,Guo, Lei.,Xie, Jianwei.,...&He, Zhike.(2006).In vitro selection of DNA aptamer against abrin toxin and aptamer-based abrin direct detection.. |
| MLA | Tang, Jijun,et al."In vitro selection of DNA aptamer against abrin toxin and aptamer-based abrin direct detection".(2006). |
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