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	A Surface Energy Transfer Nanoruler for Measuring Binding Site Distances on Live Cell Surfaces
	Chen, Yan（Univ Florida, Ctr Res Bio Nano Interface, Dept Chem） ; O'Donoghue, Meghan B.（Univ Florida, Ctr Res Bio Nano Interface, Dept Chem） ; Huang, Yu-Fen（Univ Florida, Ctr Res Bio Nano Interface, Dept Chem） ; Kang, Huaizhi（Univ Florida, Ctr Res Bio Nano Interface, Dept Chem） ; Phillips, Joseph A.（（Univ Florida, Ctr Res Bio Nano Interface, Dept Chem）） ; Chen, Xiaolan ; Chen XL(陈小兰) ; Estevez, M. -Carmen（Univ Florida, Ctr Res Bio Nano Interface, Dept Chem） ; Yang, Chaoyong ; Yang CY(杨朝勇) ; Tan, Weihong（Univ Florida, Ctr Res Bio Nano Interface, Dept Chem）
	2010-11
英文摘要	Measuring distances at molecular length scales in living systems is a significant challenge. Methods like Forster resonance energy transfer (FRET) have limitations due to short detection distances and strict oriental ions. Recently, surface energy transfer (SET) has been used in bulk solutions; however, it cannot be applied to living systems. Here, we have developed an SET nanoruler, using aptamer-gold nanoparticle conjugates with different diameters, to monitor the distance between binding sites of a receptor on living cells. The nanoruler can measure separation distances well beyond the detection limit of FRET. Thus, for the firs: time, we have developed an effective SET nanoruler for live cells with long distance, easy construction, fast detection, and low background. This is also the first time that the distance between the aptamer and antibody binding sites in the membrane protein PTK7 was measured accurately. The SET nanoruler represents the next leap forward to monitor structural components within living cell membranes.; Chinese Government ; Academic Women Madelyn Lockhart Dissertation Fellowship ; NIH ; NSF ; National Basic Research Program of China [2007CB935603, 2010CB732402]; [2009ZX10004-312]
语种	英语
出版者	AMER CHEMICAL SOC
内容类型	期刊论文
源URL	[http://pubs.acs.org/doi/abs/10.1021/ja106360v]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Chen, Yan,O'Donoghue, Meghan B.,Huang, Yu-Fen,et al. A Surface Energy Transfer Nanoruler for Measuring Binding Site Distances on Live Cell Surfaces[J],2010.
APA	Chen, Yan.,O'Donoghue, Meghan B..,Huang, Yu-Fen.,Kang, Huaizhi.,Phillips, Joseph A..,...&Tan, Weihong.(2010).A Surface Energy Transfer Nanoruler for Measuring Binding Site Distances on Live Cell Surfaces..
MLA	Chen, Yan,et al."A Surface Energy Transfer Nanoruler for Measuring Binding Site Distances on Live Cell Surfaces".(2010).

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