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	外源激素诱导赤点石斑鱼雄性化; Masculinization of red grouper (Epinephelus akaara) induced by exogenous hormone
	洪万树 ; 张其永 ; 公茂军 ; 林伟雄 ; 上官步敏
	1994
关键词	赤点石斑鱼 17α-甲基睾酮 雄性化 Epinephelus akaara 17α-methyltestosterone masculinization
英文摘要	以每次剂量为体重的5x10-6（M/M）17α-甲基睾酮（17α-MT）投喂2~4龄赤点石斑鱼，成功地促使其提早3~4A性转变为具有生殖功能的雄性鱼。经50d投喂药饵46次，累积总剂量达到鱼体重的241.3x10-6（M/M）时，性腺中的卵巢组织萎缩，绝大部分卵母细胞已退化；精巢组织发达，间质细胞数量增多，精小囊内充满成熟精子，雄性化率达100%。实验组的流精率为93.5%。雄性化后的平均成熟系数为2.30%，与自然海区的相近似。精子寿命90MIn，活率94.6%。激素诱导的精液与催产诱导雌性鱼成熟卵子进行人工授精，受精率81.1%，胚胎发育正常，受精后23H30MIn孵出仔鱼，孵化率76.7%。证实了经外源激素诱导雄性化的赤点石斑鱼精子具有受精能力。; Two to Four-year-old Females of red grouper (Epinephelus akaara ) were successFully induced to Functional males and the rate reached 100%, with oral application of 17α-methyltestosterone (17α-MT) at the dosage of 5×α10-6 (m/m ) over a 50d duration, The sex reversal ages of treated animals were 3～4 years earlier than those of the natural indlviduals.As the accumulative dosage of 17α-MT reached.241.3 × 10-6 (m/m ), it was histologically observed that ovarian tissues atrophied and most oocytes degenerated,whereas testicular tissues developed well and some seminiFerous cysts Filled with mature sperms.The number of leydig' s cells increased with the development of testicular tissues.In the experimental group semen Flowed out From 29 individuals by squeezing the belly of 31 Functionalmales.AFter masculinization the mean value of gonadosomatic index (GSI ) For the treated Fishes was 2.30%, which was the same as that For natural ones.The mean activity rate of sperms reached 94.6% and the sperm movement stopped aFter 90 minutes.ArtiFicial Fertilization was carrled out by squeezed semen Form masculinized animals and a Fertilization rate of 81.1 % was obtained.Embryonic development underwent normally and larvae began hatching by 23 hours and 30 minutes aFter insemination,with a hatching rate of 76.7%.The results of this study have solved the shortage and uncertain supply mature males in the larval rearing of the red grouper.; 国家自然科学基金
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/105027]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	洪万树,张其永,公茂军,等. 外源激素诱导赤点石斑鱼雄性化, Masculinization of red grouper (Epinephelus akaara) induced by exogenous hormone[J],1994.
APA	洪万树,张其永,公茂军,林伟雄,&上官步敏.(1994).外源激素诱导赤点石斑鱼雄性化..
MLA	洪万树,et al."外源激素诱导赤点石斑鱼雄性化".(1994).

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