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	鬼鲉肝脏提取物对小鼠免疫功能的影响; Influence of Inimicusjaponicasliver extract on mice's immune function
	葛雅辉 ; 刘欣 ; 唐于平 ; 郭建明 ; 丁少雄 ; 林祥志 ; 林汝榕 ; 段金廒
	2014-12-15
关键词	鬼鲉 肝脏提取物 体液免疫 细胞免疫 细胞因子 Inimicus japonicas liver extract humoral immunity cytoimmunity cytokine
英文摘要	目的研究鬼鲉肝脏提取物对免疫低下小鼠免疫功能的影响。方法采用环磷酰胺诱导建立免疫低下动物模型,免疫器官重量法测定胸腺指数和脾脏指数,碳粒廓清法测定小鼠单核巨噬细胞的功能,绵羊红细胞免疫法测定小鼠体液免疫功能,采用血细胞计数仪检测小鼠外周血常规,采用ElISA方法测定小鼠血清中Tnf-α、Il-1β、Il-2、Il-6的水平,采用流式细胞仪测定脾细胞周期。结果鬼鲉肝脏提取物可以减缓免疫低下小鼠体重的下降趋势以及脾脏和胸腺的损伤、促进其脾细胞的分裂和增殖、增加其单核巨噬细胞吞噬功能和血清溶血素含量以及白细胞数、中性粒细胞数、淋巴细胞数和单核细胞数和血清中Tnf-α、Il-1β、Il-2、Il-6的含量。结论鬼鲉肝脏提取物可以增强免疫低下小鼠的免疫功能。; Objective To investigate the influence of Inimicus japonicas liver extract on the immune function of immunosuppressed mice.Methods Immunosuppression animal model was established by cyclophosphamide induction.The immunity organs were weighed to measure the thymus index and spleen index;the clearance of charcoal particles method was used to measure mice's mononuclear macrophages functions,and sheep red blood cells immunizations was used to measure mice's humoral immune functions;mice's blood counts were measured by blood-counter system,and Enzyme-linked Immuno Sorbent Assay(ELISA)was used to measured the TNF-α,IL-1β,IL-2and IL-6level in blood serum;the spleen cell cycle was measured by flow cytometry.Results Inimicus japonicas liver extract significantly improved the thymus index and spleen index,promoted the division and proliferation of spleen cells,improved the mononuclear macrophages functions,increased the hemolysin level of blood serum and the levels of leukocytes,neutrophils,lymphocytes and monocytes,and also increased the levels of TNF-α,IL-1β,IL-2and IL-6in blood serum.Conclusion Inimicus japonicas liver extract enhanced immunosuppressed mice's immune function.; 海洋公益性行业科研专项(201205024-1)资助
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/104716]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	葛雅辉,刘欣,唐于平,等. 鬼鲉肝脏提取物对小鼠免疫功能的影响, Influence of Inimicusjaponicasliver extract on mice's immune function[J],2014.
APA	葛雅辉.,刘欣.,唐于平.,郭建明.,丁少雄.,...&段金廒.(2014).鬼鲉肝脏提取物对小鼠免疫功能的影响..
MLA	葛雅辉,et al."鬼鲉肝脏提取物对小鼠免疫功能的影响".(2014).

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