QQ客服 官方微博 反馈留言

	僧帽牡蛎EGFR基因的原核表达及其在幼体附着变态过程中的表达特性; Prokaryotic Expression and mRNA Expression Analysis of Epidermal Growth Factor Receptor During Attachment and Metamorphosis from Crassostrea gigas angulata
	韩国栋 ; 杨丙晔 ; 秦骥 ; 柯才焕
	2012
关键词	僧帽牡蛎 EGFR 原核表达 附着 变态 Crassostrea gigas angulata EGFR prokaryotic expression attachment metamorphosis
英文摘要	扩增僧帽牡蛎(CrASSOSTrEA gIgAS AngulATA)的表皮生长因子受体(EPIdErMAl grOWTH fACTOr rECEPTOr,Egfr)基因,并与PET32A(+)载体连接,构建PET32A(+)/Egfr重组质粒.将重组质粒导入大肠杆菌(ESCHErICHIA COlI)bl21(dE3),成功地表达并纯化了一个50ku的重组蛋白.采用rT-PCr技术,检测了僧帽牡蛎幼体附着变态前后Egfr基因表达量的变化,结果表明,Egfr基因在附着变态前的浮游幼体阶段和附着变态24H后的稚贝阶段低表达,附着后1~6H强烈表达,这预示着Egfr基因在僧帽牡蛎幼体附着变态这一生物学过程中具有重要作用.; Most benthic marine invertebrates have pelagic larvae that develop to competent stage and then settle on the bottom in response to physical,biological and chemical factors.Many previous investigators focused on exploring the inductive effects of chemical cues on larval settlement and metamorphosis.In this study,a ORF of epidermal growth factor receptor(EGFR) was amplified from sengmao oyster,Crassostrea gigas angulata and the target gene was cloned into prokaryotic expressing vector,pET-32a.After transformation,a soluble protein fused with a hexahistidine affinity ligand was expressed in E.coli BL21(DE3).The 50 ku recombinant protein was highly purified using affinity chromatography.As demonstrated by RT-PCR,EGFR was strongly expressed after 1 to 6 h of attachment of sengmao oyster larvae and weekly expressed before attachment and after 24 h of metamorphosis.And the result of RT-PCR indicated that EGFR played an important role during attachment and metamorphosis of sengmao oyster larvae.; 国家重点基础研究发展计划(973)项目(2010CB126403)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/104514]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	韩国栋,杨丙晔,秦骥,等. 僧帽牡蛎EGFR基因的原核表达及其在幼体附着变态过程中的表达特性, Prokaryotic Expression and mRNA Expression Analysis of Epidermal Growth Factor Receptor During Attachment and Metamorphosis from Crassostrea gigas angulata[J],2012.
APA	韩国栋,杨丙晔,秦骥,&柯才焕.(2012).僧帽牡蛎EGFR基因的原核表达及其在幼体附着变态过程中的表达特性..
MLA	韩国栋,et al."僧帽牡蛎EGFR基因的原核表达及其在幼体附着变态过程中的表达特性".(2012).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。