The role of hybridization in improving the immune response and thermal tolerance of abalone

CORC > 厦门大学 > 海洋环境－已发表论文

	The role of hybridization in improving the immune response and thermal tolerance of abalone
	Liang, Shuang ; Luo, Xuan ; You, Weiwei ; Luo, Lianzhong ; Ke, Caihuan ; Luo X(骆轩) ; You WW(游伟伟) ; Luo LZ(罗联忠) ; Ke CH(柯才焕)
刊名	http://dx.doi.org/10.1016/j.fsi.2014.04.014
	2014
关键词	HALIOTIS-DIVERSICOLOR-SUPERTEXTA DISCUS-HANNAI INO VIBRIO-PARAHAEMOLYTICUS WATER TEMPERATURE CHLAMYS-FARRERI GENE-EXPRESSION APOSTICHOPUS-JAPONICUS MOLLUSCAN AQUACULTURE LITOPENAEUS-VANNAMEI PENAEUS-MONODON
英文摘要	Hi-Tech Research and Development (863) Program of China [2012AA10A412]; National Natural Science Foundation of China [41106120]; Fujian Science and Technology Program [2012N5012]; Guangdong Province - Department of Education of China Joint Project [2012B091100085]; Earmarked Fund for Modern Agro-industry Technology Research System [CARS-48]; Recently, frequent death of cultured abalone drew our attention to the stress tolerance of abalone. Hybridization is an effective way of genetic improvement in aquaculture, which can introduce improved traits to the hybrids. In this study, we challenged the hybrids between Haliotis discus hannai and Haliotis gigantea, and their parents with bacteria (vibrio harveyi, vibrio alginolyticus and vibrio parahemolyticus), then held them at 20 degrees C and 28 degrees C, survival rates of the parental populations and hybrid populations were recorded. Then we tested the immune responses and thermal-induced responses of the four populations at different temperatures. Total hemocyte count (THC), respiratory burst, superoxide dismutase activity (SOD), acid phosphatase activity (ACP), alkaline phosphatase activity (AKP), myeloperoxidase activity (MPO), and HSP70 expression were determined on day 1 and day 7 of the temperature exposure. Results showed higher survival rates of the hybrids than their parents against bacteria challenge. For immune parameters, THCs were evaluated at 28 degrees C, while increased THC was also observed in H. discus hannai female x H. gigantea male (DG) and H. discus hannai female x H. discus hannai male (DD) at 12 degrees C (day 7); at 28 degrees C, respiratory burst was activated (day 1 and 7), while SOD activity first rose then fell over 7-days exposure; AKP activity was elevated at 12 degrees C and 28 degrees C (day 1), most notably in DG, and an increased level of ACP was observed in DG at 28 degrees C (day 7); MPO activity was suppressed at 12 degrees C and 28 degrees C on day 1, but recovered on day 7. For HSP70, increased HSP70 levels were observed in all populations at 28 degrees C (day 1), and DD got the lowest HSP70 level after 7-days exposure at 28 degrees C. Overall, the results suggest that temperature changes could significantly affect the physiological status of abalone, and hybrids may be more resistant to disease and thermal stresses than their parents. (C) 2014 Elsevier Ltd. All rights reserved.
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88106]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Liang, Shuang,Luo, Xuan,You, Weiwei,et al. The role of hybridization in improving the immune response and thermal tolerance of abalone[J]. http://dx.doi.org/10.1016/j.fsi.2014.04.014,2014.
APA	Liang, Shuang.,Luo, Xuan.,You, Weiwei.,Luo, Lianzhong.,Ke, Caihuan.,...&柯才焕.(2014).The role of hybridization in improving the immune response and thermal tolerance of abalone.http://dx.doi.org/10.1016/j.fsi.2014.04.014.
MLA	Liang, Shuang,et al."The role of hybridization in improving the immune response and thermal tolerance of abalone".http://dx.doi.org/10.1016/j.fsi.2014.04.014 (2014).