Cloning and expression of prostaglandin E-2 receptor subtype 1 (ep (1) ) in Bostrichthys sinensis

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	Cloning and expression of prostaglandin E-2 receptor subtype 1 (ep (1) ) in Bostrichthys sinensis
	Lai, Xiao Jian ; Hong, Wan Shu ; Liu, Fang ; Zhang, Yu Ting ; Chen, Shi Xi ; Hong WS(洪万树) ; Chen SX(陈仕玺)
刊名	http://dx.doi.org/10.1007/s10695-014-9923-x
	2014
关键词	SEX-PHEROMONE OLFACTORY SENSITIVITY PROSTANOID RECEPTORS GOLDFISH SALMON FISH CARP
英文摘要	National Natural Science Foundation of China [41276129, 40776080]; fund for Doctor Station of the Ministry of China [20120121110029]; Major Project of Agricultural Science and Technology of Fujian Province [2012N5011]; Our previous studies suggested that prostaglandin E-2 (PGE(2)) is a putative sex pheromone in Chinese black sleeper Bostrichthys sinensis, a fish species that inhabits intertidal zones and mates and spawns inside a muddy burrow. We found immunoreactivities of PGE(2) receptor subtypes (Ep(1-3)) expressed in the olfactory sac, but only Ep(1) presented higher density of immunoreactivity in mature fish than that in immature fish in both sexes. To gain a better understanding of the underlying molecular mechanism for the detection of PGE(2) in the olfactory system, we cloned an ep (1) cDNA from the adult olfactory sac. The open-reading frame of the ep (1) consisted of 1,134-bp nucleotides that encoded a 378-amino acid-long protein with a seven-transmembrane domain, typical for the G protein-coupled receptors superfamily. Expression of ep (1) mRNA was observed in all tissues examined, with higher levels obtained in the olfactory sacs and testes. The expression of ep (1) mRNA in the olfactory sacs and gonads was significantly higher in both sexes of mature fish than in those of immature ones. Taken together, our results suggested that Ep(1), which is highly expressed in the olfactory sacs and gonads of mature fish, is important for the control of reproduction and may be involved in PGE(2)-initiated spawning behavior in B. sinensis.
语种	英语
出版者	SPRINGER
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88093]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Lai, Xiao Jian,Hong, Wan Shu,Liu, Fang,et al. Cloning and expression of prostaglandin E-2 receptor subtype 1 (ep (1) ) in Bostrichthys sinensis[J]. http://dx.doi.org/10.1007/s10695-014-9923-x,2014.
APA	Lai, Xiao Jian.,Hong, Wan Shu.,Liu, Fang.,Zhang, Yu Ting.,Chen, Shi Xi.,...&陈仕玺.(2014).Cloning and expression of prostaglandin E-2 receptor subtype 1 (ep (1) ) in Bostrichthys sinensis.http://dx.doi.org/10.1007/s10695-014-9923-x.
MLA	Lai, Xiao Jian,et al."Cloning and expression of prostaglandin E-2 receptor subtype 1 (ep (1) ) in Bostrichthys sinensis".http://dx.doi.org/10.1007/s10695-014-9923-x (2014).