A covalent reporter of β-lactamase activity for fluorescent imaging and rapid screening of antibiotic-resistant bacteria

CORC > 厦门大学 > 海洋环境－已发表论文

	A covalent reporter of β-lactamase activity for fluorescent imaging and rapid screening of antibiotic-resistant bacteria
	Shao, Qing ; Zheng, Yan ; Dong, Xueming ; Tang, Kai ; Yan, Xiaomei ; Xing, Bengang ; Tang K(汤凯)
刊名	http://dx.doi.org/10.1002/chem.201301654
	2013-08-12
关键词	Antibiotics Electrophoresis Energy transfer Enzymes Fluorescence Mass spectrometry Probes Testing
英文摘要	Bacterial resistance to antibiotics poses a great clinical challenge in fighting serious infectious diseases due to complicated resistant mechanisms and time-consuming testing methods. Chemical reaction-directed covalent labeling of resistance-associated bacterial proteins in the context of a complicated environment offers great opportunity for the in-depth understanding of the biological basis conferring drug resistance, and for the development of effective diagnostic approaches. In the present study, three fluorogenic reagents LRBL1-3 for resistant bacteria labeling have been designed and prepared on the basis of fluorescence resonance energy transfer (FRET). The hydrolyzed probes could act as reactive electrophiles to attach the enzyme, β-lactamase, and thus facilitated the covalent labeling of drug resistant bacterial strains. SDS electrophoresis and MALDI-TOF mass spectrometry characterization confirmed that these probes were sensitive and specific to β-lactamase and could therefore serve for covalent and localized fluorescence labeling of the enzyme structure. Moreover, this β-lactamase-induced covalent labeling provides quantitative analysis of the resistant bacterial population (down to 5 %) by high resolution flow cytometry, and allows single-cell detection and direct observation of bacterial enzyme activity in resistant pathogenic species. This approach offers great promise for clinical investigations and microbiological research. ? 2013 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
语种	英语
出版者	Wiley-VCH Verlag
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88078]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Shao, Qing,Zheng, Yan,Dong, Xueming,et al. A covalent reporter of β-lactamase activity for fluorescent imaging and rapid screening of antibiotic-resistant bacteria[J]. http://dx.doi.org/10.1002/chem.201301654,2013.
APA	Shao, Qing.,Zheng, Yan.,Dong, Xueming.,Tang, Kai.,Yan, Xiaomei.,...&汤凯.(2013).A covalent reporter of β-lactamase activity for fluorescent imaging and rapid screening of antibiotic-resistant bacteria.http://dx.doi.org/10.1002/chem.201301654.
MLA	Shao, Qing,et al."A covalent reporter of β-lactamase activity for fluorescent imaging and rapid screening of antibiotic-resistant bacteria".http://dx.doi.org/10.1002/chem.201301654 (2013).