Molecular cloning and expression analysis of major histocompatibility complex class IIB gene of the Whitespotted bambooshark (Chiloscyllium plagiosum)

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	Molecular cloning and expression analysis of major histocompatibility complex class IIB gene of the Whitespotted bambooshark (Chiloscyllium plagiosum)
	Ma, Qian ; Su, Yong-Quan ; Wang, Jun ; Zhuang, Zhi-Meng ; Tang, Qi-Sheng ; Wang J(王军) ; Tang QS(唐启升)
刊名	http://dx.doi.org/10.1007/s10695-012-9685-2
	2013
关键词	SALMON SALMO-SALAR MHC CLASS-I ATLANTIC SALMON NURSE SHARK GINGLYMOSTOMA-CIRRATUM HEAD KIDNEY BETA-CHAIN TRANSSPECIES POLYMORPHISM SEQUENCE-ANALYSIS VIBRIO-HARVEYI
英文摘要	National Natural Science Foundation of China [40876080]; Major histocompatibility complex (MHC) plays an important role in the immune response to antigenic peptides in vertebrates. In this study, the full length of MHC IIB cDNA was isolated from the Whitespotted bambooshark (Chiloscyllium plagiosum) by homology cloning, and the rapid amplification of cDNA ends polymerase chain reaction. As a result, the MHC IIB cDNA is 1,407 bp, which contains an open reading frame (ORF) of 831 bp encoding a protein of 276 amino acids. Furthermore, seven alleles of the complete MHC IIB ORF were detected and the variable sites were mainly located in the immunoglobulin-like (beta(2)) region. Tissue distribution analysis showed that MHC IIB can be detected in all the ten tissues examined, with the highest expression in the spleen and gill. Challenge of C. plagiosum with the pathogenic bacteria, Vibrio harveyi, resulted in significant changes in the expression of MHC IIB mRNA in the three immune-related tissues (gill, liver and spleen). These results show that the MHC IIB plays an important role in response to bacterial infection in elasmobranches.
语种	英语
出版者	SPRINGER
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88059]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Ma, Qian,Su, Yong-Quan,Wang, Jun,et al. Molecular cloning and expression analysis of major histocompatibility complex class IIB gene of the Whitespotted bambooshark (Chiloscyllium plagiosum)[J]. http://dx.doi.org/10.1007/s10695-012-9685-2,2013.
APA	Ma, Qian.,Su, Yong-Quan.,Wang, Jun.,Zhuang, Zhi-Meng.,Tang, Qi-Sheng.,...&唐启升.(2013).Molecular cloning and expression analysis of major histocompatibility complex class IIB gene of the Whitespotted bambooshark (Chiloscyllium plagiosum).http://dx.doi.org/10.1007/s10695-012-9685-2.
MLA	Ma, Qian,et al."Molecular cloning and expression analysis of major histocompatibility complex class IIB gene of the Whitespotted bambooshark (Chiloscyllium plagiosum)".http://dx.doi.org/10.1007/s10695-012-9685-2 (2013).