A Covalent Reporter of -Lactamase Activity for Fluorescent Imaging and Rapid Screening of Antibiotic-Resistant Bacteria

CORC > 厦门大学 > 海洋环境－已发表论文

	A Covalent Reporter of -Lactamase Activity for Fluorescent Imaging and Rapid Screening of Antibiotic-Resistant Bacteria
	Shao, Qing ; Zheng, Yan ; Dong, Xueming ; Tang, Kai ; Yan, Xiaomei ; Xing, Bengang ; Tang K(汤凯) ; Yan XM(颜晓梅)
刊名	http://dx.doi.org/10.1002/chem.201301654
	2013
关键词	BETA-LACTAMASE REAL-TIME IN-VIVO STAPHYLOCOCCUS-AUREUS CELL-WALL GENE-EXPRESSION LIVING CELLS PROTEIN ENZYME CHEMISTRY
英文摘要	NSF of P.R. China [21225523, 21027010, 20975087, 90913015]; MOE; Nanyang Technological University, Singapore [RG 64/10]; Bacterial resistance to antibiotics poses a great clinical challenge in fighting serious infectious diseases due to complicated resistant mechanisms and time-consuming testing methods. Chemical reaction-directed covalent labeling of resistance-associated bacterial proteins in the context of a complicated environment offers great opportunity for the in-depth understanding of the biological basis conferring drug resistance, and for the development of effective diagnostic approaches. In the present study, three fluorogenic reagents LRBL1-3 for resistant bacteria labeling have been designed and prepared on the basis of fluorescence resonance energy transfer (FRET). The hydrolyzed probes could act as reactive electrophiles to attach the enzyme, beta-lactamase, and thus facilitated the covalent labeling of drug resistant bacterial strains. SDS electrophoresis and MALDI-TOF mass spectrometry characterization confirmed that these probes were sensitive and specific to b-lactamase and could therefore serve for covalent and localized fluorescence labeling of the enzyme structure. Moreover, this beta-lactamase-induced covalent labeling provides quantitative analysis of the resistant bacterial population (down to 5%) by high resolution flow cytometry, and allows single-cell detection and direct observation of bacterial enzyme activity in resistant pathogenic species. This approach offers great promise for clinical investigations and microbiological research.
语种	英语
出版者	WILEY-V C H VERLAG GMBH
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/87984]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Shao, Qing,Zheng, Yan,Dong, Xueming,et al. A Covalent Reporter of -Lactamase Activity for Fluorescent Imaging and Rapid Screening of Antibiotic-Resistant Bacteria[J]. http://dx.doi.org/10.1002/chem.201301654,2013.
APA	Shao, Qing.,Zheng, Yan.,Dong, Xueming.,Tang, Kai.,Yan, Xiaomei.,...&颜晓梅.(2013).A Covalent Reporter of -Lactamase Activity for Fluorescent Imaging and Rapid Screening of Antibiotic-Resistant Bacteria.http://dx.doi.org/10.1002/chem.201301654.
MLA	Shao, Qing,et al."A Covalent Reporter of -Lactamase Activity for Fluorescent Imaging and Rapid Screening of Antibiotic-Resistant Bacteria".http://dx.doi.org/10.1002/chem.201301654 (2013).