QQ客服 官方微博 反馈留言

	Development of a PNA probe for the detection of the toxic dinoflagellate Takayama pulchella
	Huang, Bangqin ; Huang BQ(黄邦钦) ; Hou, Jianjun ; Lin, Senjie（Univ Connecticut, Dept Marine Sci, Groton, CT 06340 USA） ; Chen, Jixin ; Hong, Huasheng
	2008-06
关键词	fluorescent in situ hybridization (FISH) harmful algal blooms (HABs) molecular probes peptide nucleic acid (PNA) Takayama pulchella TPXM
英文摘要	A peptide nucleic acid (PNA) probe was developed to detect the toxic dinoflagellate, Takayama pulchella TPXM, using fluorescent in situ hybridization (FISH) combined with epifluorescent microscopy and flow cytometry. The PNA probe was then used to analyze HAB samples from Xiamen Bay. The results indicated that the fluorescein phosphoramidite (FAM)-labeled probe (PNATP28S01) [Flu]-OO ATG CCA TCT CAA GA, entered the algal cells easily and bound to the target species specifically. High hybridization efficiency (nearly 100%) was observed. Detection by epifluorescence microscopy and flow cytometry gave comparable results. The fluorescence intensity of the PNA probe hybridized to T pulchella cells was remarkably higher than that of two DNA probes used in this study and than the autofluorescence of the blank and negative control cells. In addition, the hybridization condition of the PNA probe was easier to control than DNA probes, and when applied to field-collected samples, the PNA probe showed higher binding efficiency to the target species than DNA probes. With the observed high specificity, binding efficiency, and detection signal intensity, the PNA probe will be useful for monitoring harmful algal blooms of T pulchella. (C) 2008 Published by Elsevier B.V.
语种	英语
出版者	ELSEVIER SCIENCE BV
内容类型	期刊论文
源URL	[http://dx.doi.org/doi: 10.1016/j.hal.2007.11.006]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Huang, Bangqin,Huang BQ,Hou, Jianjun,et al. Development of a PNA probe for the detection of the toxic dinoflagellate Takayama pulchella[J],2008.
APA	Huang, Bangqin,黄邦钦,Hou, Jianjun,Lin, Senjie,Chen, Jixin,&Hong, Huasheng.(2008).Development of a PNA probe for the detection of the toxic dinoflagellate Takayama pulchella..
MLA	Huang, Bangqin,et al."Development of a PNA probe for the detection of the toxic dinoflagellate Takayama pulchella".(2008).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。