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	Identification and Characterization of Full-Length cDNAs in Channel Catfish (Ictalurus punctatus) and Blue Catfish (Ictalurus furcatus)
	Chen, Fei ; Lee, Yoona（Auburn Univ, Dept Fisheries & Allied Aquacultures, Aquat Genom Unit, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA） ; Jiang, Yanliang（Auburn Univ, Dept Fisheries & Allied Aquacultures, Aquat Genom Unit, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA） ; Wang, Shaolin（Auburn Univ, Dept Fisheries & Allied Aquacultures, Aquat Genom Unit, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA） ; Peatman, Eric（Auburn Univ, Dept Fisheries & Allied Aquacultures, Aquat Genom Unit, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA） ; Abernathy, Jason（Auburn Univ, Dept Fisheries & Allied Aquacultures, Aquat Genom Unit, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA） ; Liu, Hong（Auburn Univ, Dept Fisheries & Allied Aquacultures, Aquat Genom Unit, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA） ; Liu, Shikai（Auburn Univ, Dept Fisheries & Allied Aquacultures, Aquat Genom Unit, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA） ; Kucuktas, Huseyin（Auburn Univ, Dept Fisheries & Allied Aquacultures, Aquat Genom Unit, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA） ; Ke, Caihuan ; Ke CH(柯才焕) ; Liu, Zhanjiang（Auburn Univ, Dept Fisheries & Allied Aquacultures, Aquat Genom Unit, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA）
	2010-07-12
英文摘要	Background: Genome annotation projects, gene functional studies, and phylogenetic analyses for a given organism all greatly benefit from access to a validated full-length cDNA resource. While increasingly common in model species, full-length cDNA resources in aquaculture species are scarce. Methodology and Principal Findings: Through in silico analysis of catfish (Ictalurus spp.) ESTs, a total of 10,037 channel catfish and 7,382 blue catfish cDNA clones were identified as potentially encoding full-length cDNAs. Of this set, a total of 1,169 channel catfish and 933 blue catfish full-length cDNA clones were selected for re-sequencing to provide additional coverage and ensure sequence accuracy. A total of 1,745 unique gene transcripts were identified from the full-length cDNA set, including 1,064 gene transcripts from channel catfish and 681gene transcripts from blue catfish, with 416 transcripts shared between the two closely related species. Full-length sequence characteristics (ortholog conservation, UTR length, Kozak sequence, and conserved motifs) of the channel and blue catfish were examined in detail. Comparison of gene ontology composition between full-length cDNAs and all catfish ESTs revealed that the full-length cDNA set is representative of the gene diversity encoded in the catfish transcriptome. Conclusions: This study describes the first catfish full-length cDNA set constructed from several cDNA libraries. The catfish full-length cDNA sequences, and data gleaned from sequence characteristics analysis, will be a valuable resource for ongoing catfish whole-genome sequencing and future gene-based studies of function and evolution in teleost fishes.; United States Department of Agriculture National Institute for Food and Agriculture Animal Genome Basic Genome Reagents and Tools Program [2009-35205-05101]; Korean Government (MOEHRD) [KRF-2008-357-F00035]
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1371/journal.pone.0011546]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Chen, Fei,Lee, Yoona,Jiang, Yanliang,et al. Identification and Characterization of Full-Length cDNAs in Channel Catfish (Ictalurus punctatus) and Blue Catfish (Ictalurus furcatus)[J],2010.
APA	Chen, Fei.,Lee, Yoona.,Jiang, Yanliang.,Wang, Shaolin.,Peatman, Eric.,...&Liu, Zhanjiang.(2010).Identification and Characterization of Full-Length cDNAs in Channel Catfish (Ictalurus punctatus) and Blue Catfish (Ictalurus furcatus)..
MLA	Chen, Fei,et al."Identification and Characterization of Full-Length cDNAs in Channel Catfish (Ictalurus punctatus) and Blue Catfish (Ictalurus furcatus)".(2010).

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