Probe droplet arrays generated in the capillary for microarray analysis

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	Probe droplet arrays generated in the capillary for microarray analysis
	Qu, Xiangmeng ; Wang, Yuqiao ; Shi, Zhan ; Fu, Guangchun ; Zeng, Xiang ; Li, Xin ; Chen, Hong ; Shi Z(施展)
刊名	http://dx.doi.org/10.1016/j.bios.2012.06.022
关键词	MICROFLUIDIC CHIP DNA MICROARRAYS INJECTION-ANALYSIS THIN-FILMS HYBRIDIZATION DEVICE FLOW TECHNOLOGY GLASS
英文摘要	National Natural Science Foundation of China [21005066]; Doctoral Fund of Ministry of Education of China [20100121120025]; Fundamental Research Funds for the Central Universities [2010121043, 2010121044]; Microarray technology is a useful tool for nucleic acid detection and has been widely used in biology and related research fields. However, the procedure is labor intensive and time consuming. Microfluidic chip-based microarrays save time with better performance, but the low spot density and probe number limit its applications. To develop high performance microarrays with high spot density within a microchannel, a method is reported here for preparing microarrays in a capillary by generating probe droplet arrays. The probes in droplets are immobilized onto the inner wall of the capillary to form a one-dimensional probe array, and then a sample solution is introduced to hybridize with the probe array. The effect of the capillary's inner diameter was evaluated to realize a high-density probe array. The processes of array generation and probe immobilization were studied to avoid possible cross contamination. The background from probe immobilization during the array generation and incubation was quantified to assure sensitivity. Multiple sample detection was also demonstrated within one capillary. The capillary based microarray assay had high spot density, easy fabrication, fast detection, high sensitivity and multiple sample capacity. (C) 2012 Elsevier B.V. All rights reserved.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/59551]
专题	材料学院－已发表论文
推荐引用方式 GB/T 7714	Qu, Xiangmeng,Wang, Yuqiao,Shi, Zhan,et al. Probe droplet arrays generated in the capillary for microarray analysis[J]. http://dx.doi.org/10.1016/j.bios.2012.06.022.
APA	Qu, Xiangmeng.,Wang, Yuqiao.,Shi, Zhan.,Fu, Guangchun.,Zeng, Xiang.,...&施展.
MLA	Qu, Xiangmeng,et al."Probe droplet arrays generated in the capillary for microarray analysis".http://dx.doi.org/10.1016/j.bios.2012.06.022