东海原甲藻钙调蛋白的分离纯化及鉴定

CORC > 集美大学

	东海原甲藻钙调蛋白的分离纯化及鉴定
	郭彩华 ; 刘静雯 ; 卢珍华 ; 詹玲玲
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200901005&dbcode=CJFQ&dbname=CJFQ2009
	2012-06-05 ; 2012-06-05
关键词	东海原甲藻钙调蛋白疏水纯化 Prorocentrum donghaiense Lu calmodulin(CaM) hydrophobic chromatography Q946
其他题名	Purification and Identification of Calmodulin(CaM) from Prorocentrum donghaiense Lu
中文摘要	以东海原甲藻(Prorocentrum donghaienseLu)为材料,经热变性、离心处理,上清液依次经DEAE纤维素阴离子交换层析和苯基-Sepharose CL-4B疏水层析分离纯化钙调蛋白(CaM).SDS-PAGE和Western blot检测结果显示:东海原甲藻中CaM的相对分子质量为16 ku.采用该实验方法首次有效地从海洋浮游植物东海原甲藻中分离纯化出钙调蛋白.; Calmodulin(CaM) was extracted and purified from marine phytoplankton Prorocentrum donghaiense Lu cells.The supernatant dealt with the thermal denaturation and the freeze-centrifugation was purified by the diethylaminoethyl(DEAE) cellulose ion exchange chromatography and the phenyl-sepharose CL-4B hydrophobic chromatography sequentially.CaM was detected by using SDS-PAGE and western blot.The results showed that the relative molecular mass of CaM in P.donghaiense was 16 ku and the procedures used in this experiment were effective for extraction and purification of CaM from marine phytoplankton.; 【作者单位】集美大学生物工程学院；【作者英文名】GUO Cai-hua,LIU Jing-wen,LU Zhen-hua,ZHAN Ling-ling(School of Biotechnology Engineering,Jimei University,Xiamen 361021,China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18627]
专题	集美大学
推荐引用方式 GB/T 7714	郭彩华,刘静雯,卢珍华,等. 东海原甲藻钙调蛋白的分离纯化及鉴定[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200901005&dbcode=CJFQ&dbname=CJFQ2009,2012, 2012.
APA	郭彩华,刘静雯,卢珍华,&詹玲玲.(2012).东海原甲藻钙调蛋白的分离纯化及鉴定.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200901005&dbcode=CJFQ&dbname=CJFQ2009.
MLA	郭彩华,et al."东海原甲藻钙调蛋白的分离纯化及鉴定".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200901005&dbcode=CJFQ&dbname=CJFQ2009 (2012).