‘SK4-316’胡萝卜体胚的诱导和培养

CORC > 集美大学

	‘SK4-316’胡萝卜体胚的诱导和培养
	陈艳红 ; 刘毅君 ; 李爱贞
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=yrdt200903007&dbcode=CJFQ&dbname=CJFQ2009
	2012-06-05 ; 2012-06-05
关键词	胡萝卜体细胞胚愈伤组织 carrot somatic embryos callus S631.2
其他题名	Induction and Culture of Carrot SK4-316 Somatic Embryos
中文摘要	以‘SK4-316’胡萝卜无菌苗的下胚轴为外植体,研究不同培养基配方和培养条件对愈伤组织诱导、体细胞胚间接发生及其同步化培养的影响,以及不同脱分化时间、脱分化培养基及外植体续存时间对体细胞胚直接发生的诱导及其培养的影响。结果表明:含3%蔗糖、0.8%琼脂的1/2MS+2,4-D2.5mg/L+6-BA(或KT)0.5mg/L+CH300mg/L是诱导愈伤组织的良好培养基;1/2MS+2,4-D1.25mg/L+KT0.25mg/L+6-BA0.25mg/L(含3%蔗糖)适于愈伤组织分化并诱导体胚发生,0.02%ABA对体胚的诱导有促进作用,0.06%ABA或15%PEG能促进体胚成熟;外植体在MS+2,4-D1.0mg/L固体培养基上脱分化培养48h,再转入MS+CH300g/L液体培养基中可诱导体胚直接发生,但随着外植体续存于诱导培养基中时间的延长,体胚发生变异的几率也渐增。; The hypocotyl of carrot SK4-316 aseptic plantlets were used as explants for studying the effects of different culture medium formula and conditions on callus induction,somatic embryos and their synchronization by indirect somatic embryogenesis,and influence of different dedifferentiation time,dedifferentiation medium and the time of explants removed from induction culture systems on direct somatic embryogenesis.The results showed that the medium 1/2MS + 2,4-D2.5 mg/L + 6-BA(or KT) 0.5 mg /L + CH 300 mg/L with 3% sucrose and 0.8% agar was good for callus induction;1/2MS + 2,4-D1.25 mg/L + KT0.25 mg/L + 6-BA0.25 mg/L with 3% sucrose was optimum for somatic embryogenesis from callus,and 0.02% ABA promoted the induction of somatic embryos and 0.06% ABA or 15% PEG promoted the maturation of somatic embryos;it was suit for direct somatic embryogenesis from explants which cultured on dedifferentiation solid medium MS + 2,4-D1.0 mg/L for 48 h,and then on liquid medium MS+CH300g/L.Moreover,somatic embryos mutation rate increased gradually with culture time of explants on induction medium.; 【作者单位】集美大学生物工程学院；【作者英文名】CHEN Yan-hong,LIU Yi-jun,LI Ai-zhen(Bioengineering College,Jimei University,Xiamen 361021,Fujian China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18228]
专题	集美大学
推荐引用方式 GB/T 7714	陈艳红,刘毅君,李爱贞. ‘SK4-316’胡萝卜体胚的诱导和培养[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=yrdt200903007&dbcode=CJFQ&dbname=CJFQ2009,2012, 2012.
APA	陈艳红,刘毅君,&李爱贞.(2012).‘SK4-316’胡萝卜体胚的诱导和培养.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=yrdt200903007&dbcode=CJFQ&dbname=CJFQ2009.
MLA	陈艳红,et al."‘SK4-316’胡萝卜体胚的诱导和培养".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=yrdt200903007&dbcode=CJFQ&dbname=CJFQ2009 (2012).