饲用发酵乳酸杆菌(Lactobacillus fermentum)生物学特性的研究

CORC > 集美大学

	饲用发酵乳酸杆菌(Lactobacillus fermentum)生物学特性的研究
	黄沧海 ; 谯仕彦 ; 李德发
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jxnd200404026&dbcode=CJFQ&dbname=CJFQ2004
	2012-06-05 ; 2012-06-05
关键词	发酵乳酸杆菌生物学特性发酵参数存活率 Lactobacillus fermentum biological characteristics fermentation parameters survivability S816.3
其他题名	A Study on Biological Characteristics of Lactobacillus fermentum Applied in Feed
中文摘要	在体外研究了1株仔猪源的发酵乳酸杆菌(Lactobacillusfermentum)的生长曲线、耐热存活率、耐酸存活率、贮藏存活率等生物学特性,采用均匀设计法优化发酵乳酸杆菌的发酵培养基。结果表明,发酵乳酸杆菌的浓度在2h之后从105个数量级迅速上升,到第24h菌体浓度达到最高值,第28h之后,活菌浓度开始缓慢下降到1011数量级以下。pH2.0处理6h的存活率为80.9%,经过1个月贮藏存活率为78.4%,75℃处理15min之后存活率为45.1%。优化的发酵参数为:时间:20h;葡萄糖:10g/L;蔗糖:60g/L;胰蛋白胨:30g/L;酵母浸粉:30g/L;柠檬酸铵:12g/L。研究证明该发酵乳酸杆菌具有良好的生长繁殖性能和较强的抗逆性,在仔猪饲料中可以用作益生素菌种。; The biological characteristics of the L. fermentum originated from weaning piglet were investigated and fermentation parameters were optimized by means of uniform design. Lactobacillus growth curve, survivability after 75 ℃ treatment for 15 min, pH2.0 treatment for 6 h and storage for 1 month, respectively, were measured. The concentration of L. fermentum decreased slightly in the first hour and increased very quickly from 105 CFU/mL to 1011 CFU/mL in 24 h, but fell down under 1011 CFU/mL in 28 h. L. fermentum survivability after 75℃, storage, and pH2.0 treatment was 45.1%, 78.4%, 80.9%, respectively and fermentation parameters were as follows: culture time 20 h, glucose 10 g/L, sucrose 60 g/L, peptone 30 g/L, yeast extract 30 g/L, and ammonium citrate 12 g/L. The lactobacillus showed excellent growth characteristics and good resistance to stress so it can be used in piglet feed as a probiotics bacterium.; 【作者单位】集美大学水产学院；中国农业大学动物科技学院；中国农业大学动物科技学院福建厦门361021；北京100094；北京100094；【作者英文名】HUANG Cang-hai~1,QIAO Shi-yan~2,LI De-fa~2(1.Fisheries College, Jimei University, Xiamen 361021, China;2.College of Animal Science and Technology, CAU, Beijing 100094, China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12701]
专题	集美大学
推荐引用方式 GB/T 7714	黄沧海,谯仕彦,李德发. 饲用发酵乳酸杆菌(Lactobacillus fermentum)生物学特性的研究[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jxnd200404026&dbcode=CJFQ&dbname=CJFQ2004,2012, 2012.
APA	黄沧海,谯仕彦,&李德发.(2012).饲用发酵乳酸杆菌(Lactobacillus fermentum)生物学特性的研究.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jxnd200404026&dbcode=CJFQ&dbname=CJFQ2004.
MLA	黄沧海,et al."饲用发酵乳酸杆菌(Lactobacillus fermentum)生物学特性的研究".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jxnd200404026&dbcode=CJFQ&dbname=CJFQ2004 (2012).