鳗鲡肠道中嗜水气单胞菌的拮抗菌筛选 | |
张子华 ; 鄢庆枇 ; 邹文政 ; 邢颜丽 ; 郭松林 ; 纪荣兴 | |
刊名 | http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmwy201003003&dbcode=CJFQ&dbname=CJFQTEMP |
2012-06-05 ; 2012-06-05 | |
关键词 | 欧洲鳗鲡 嗜水气单胞菌 拮抗菌 抗菌谱 生长曲线 共凝集 Anguilla anguilla Aeromonas hydrophila antagonistic bacteria antibacterial spectra growth curves co-agglutination S941 |
其他题名 | Isolation and Screening of Antagonistic Bacteria Against Aeromonas hydrophila from Intestine of Anguilla anguilla |
中文摘要 | 从鳗鲡肠道中分离到643株细菌,用点种法筛选出65株病原性嗜水气单胞菌的拮抗菌,研究拮抗效果最好的14株拮抗菌的抗菌谱、生长曲线以及与病原性嗜水气单胞菌的共凝集作用.研究结果显示:NAC琼脂培养基筛选的2株拮抗菌(Na3-38和Nb3-15)对7株指示菌均有较强的拮抗作用,Aa3-22和Mb3-7对7株指示菌中的5株有拮抗效果;除Na3-38和Nb3-15以外,其余12株的生长系数均大于嗜水气单胞菌;11株拮抗菌(Na3-38、Nb3-15、Ab2-55、Ab2-77、Ab1-3、Aa3-22、Aa3-67、Mb1-4、Mb2-10、Mb3-7和Mb3-27)和嗜水气单胞菌的共凝集率大于10%.这表明:Aa3-22和Mb3-7在抗菌谱、生长曲线以及与病原性嗜水气单胞菌的共凝集作用都适合作为益生菌.; 643 strains were isolated from healthy Anguilla anguilla intestinal tract,65 strains of antagonistic bacteria against Aeromonas hydrophila were identified by dot-inoculating method,and 14 of the 65 strains with strong antagonistic activity were chosen for antibacterial spectra,growth curves and co-agglutination assay.The results showed:2 isolated strains(Na3-38 and Nb3-15) ,had a wider range of antibacterial spectra than others,5 of 7 indicating bacteria were obviously inhibited by isolated strains of Aa3-22 and Mb3-7;12 isolated strains had more advantage in R than A.hydrophila;the co-agglutination ratio was more than 10 % between 11 isolated strains and A.hydrophila.The results indicated that the isolated strains of Aa3-22 and Mb3-7 could be used to develop probiotics based on their antibacterial spectrum,growth curve and the co-agglutination with A.hydrophila.; 【作者单位】集美大学水产学院; 福建省高校水产科学技术与食品安全重点实验室;【作者英文名】ZHANG Zi-hua1,YAN Qing-pi1,2,ZOU Wen-zheng1,2,XING Yan-li1,2,GUO Song-lin1,2,JI Rong-xing1,2(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Science and Technology for Aquaculture and Food Safety(Jimei University) ,Fujian |
语种 | 中文 |
内容类型 | 期刊论文 |
源URL | [http://ir.calis.edu.cn/hdl/235041/12525] |
专题 | 集美大学 |
推荐引用方式 GB/T 7714 | 张子华,鄢庆枇,邹文政,等. 鳗鲡肠道中嗜水气单胞菌的拮抗菌筛选[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmwy201003003&dbcode=CJFQ&dbname=CJFQTEMP,2012, 2012. |
APA | 张子华,鄢庆枇,邹文政,邢颜丽,郭松林,&纪荣兴.(2012).鳗鲡肠道中嗜水气单胞菌的拮抗菌筛选.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmwy201003003&dbcode=CJFQ&dbname=CJFQTEMP. |
MLA | 张子华,et al."鳗鲡肠道中嗜水气单胞菌的拮抗菌筛选".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmwy201003003&dbcode=CJFQ&dbname=CJFQTEMP (2012). |
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