不同环境条件对溶藻弧菌粘附大黄鱼肠粘液的影响

CORC > 集美大学

	不同环境条件对溶藻弧菌粘附大黄鱼肠粘液的影响
	鄢庆枇 ; 陈强 ; 邹文政 ; 马甡 ; 庄峙厦 ; 王小如
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sckx200602018&dbcode=CJFQ&dbname=CJFQ2006
	2012-06-05 ; 2012-06-05
关键词	溶藻弧菌大黄鱼粘附作用粘液 Vibrio alginolyticus Pseudosciaene crocea adhesion mucus S943
其他题名	Influence of environmental factors on the adhesion of Vibrio alginolyticus to the intestinal mucus of Pseudosciaene crocea
中文摘要	研究了不同环境条件下溶藻弧菌对大黄鱼肠粘液的粘附作用。采用细菌计数法测定溶藻弧菌的粘附作用。粘附的细菌经SYBR GreenⅠ染色后在荧光显微镜下观察,用数码相机拍照后在电脑上计数。试验结果表明,溶藻弧菌对大黄鱼肠粘液的粘附量随菌浓度的升高而升高,并在1~1.5h内趋于饱和;粘附作用在15~30℃、pH偏酸时较强;盐度在5~35范围内对前肠粘液的粘附作用影响不明显,后肠粘液的粘附作用在此范围内随盐度增大而加强,在盐度为0时,溶藻弧菌对前、后肠粘液都无粘附作用;56℃热处理5min及60℃处理1h均能大幅减弱溶藻弧菌对两种肠粘液的粘附作用,表明溶藻弧菌表面的某些热敏结构在粘附作用中起着重要作用。根据以上结果,可以认为溶藻弧菌能够很好地粘附于大黄鱼肠粘液层,其粘附作用受温度、盐度、pH值等环境因子影响很大,溶藻弧菌表面的某些热敏结构可能在粘附过程中起着重要作用。研究结果表明,溶藻弧菌对大黄鱼肠粘液的粘附作用是可控制的,这对于鱼类养殖疾病的控制有一定的参考价值。; The mucus layer is the most outer defence barrier of fish.The bacterial attachment to mucus layer is a critical step in the host colonization and subsequent occurrence of infection.Adhesion of Vibrio alginolyticus to the intestinal mucus of Pseudosciaene crocea has been investigated in order to understand the characteristics and mechanisms involved in the adherence of bacteria onto fish mucus so that preventive measures can be devised to reduce infection.In vitro adhesion assay was carried out by bacterial counting.The adhesive bacteria were stained with SYBR Green Ⅰ,observed under fluorescent microscope,counted on computer display after taking pictures.The results showed that the adhesive quantity of V.alginolyticus increases with bacterial concentrations and reached equilibrium after being incubated 1-1.5 h;the higher adhesive quantity was achieved at 15-30 ℃ and acid condition;adhesion of V.alginolyticus to the mucus of foregut was not obvious variety with salinity(5-35),but adhesive quantity to the hindgut mucus enhances while salinity increases;V.alginolyticus could not adhere to intestinal mucus without salinity;adhesive quantity reduced remarkably after heat treatment of the germ(56 ℃ 5 min,60 ℃ 1 h),which indicated that heat sensitive structure of V.alginolyticus might play an important role in adhesion.The conclusions of this paper showed V.alginolyticus could adhere to intestinal mucus of P.crocea facilely in seawater;this adhesion was remarkably affected by environmental factors,such as temperature,pH and salinity;V.alginolyticus adhered to the intestinal mucus of P.crocea by specific adhesion,some heat-sensitive structures on V.alginolyticus surface played a crucial role in adherence of the bacteria to gill mucus.The results indicated that the bacterial adhesion of V.alginolyticus to intestinal mucus of P.crocea was governable.The results obtained are useful for the epidemic control in fish culture.; 【作者单位】集美大学水产学院；中国海洋大学水产学院；厦门大学化学化工学院；厦门大学化学化工学院福建厦门361021；福建厦门361005；福建厦门361021；山东青岛266003；【作者英文名】YAN Qing-pi~1,CHEN Qiang~(1,2),ZOU Wen-zheng~1,MA Shen~2,ZHUANG Zhi-xia~3,WANG Xiao-ru~3(1.Fisheries College of Jimei University,Xiamen 361021,China;2.Fisheries College,Ocean University of China,Qingdao 266003,China;3.College of Chemistry & Chemical
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12524]
专题	集美大学
推荐引用方式 GB/T 7714	鄢庆枇,陈强,邹文政,等. 不同环境条件对溶藻弧菌粘附大黄鱼肠粘液的影响[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sckx200602018&dbcode=CJFQ&dbname=CJFQ2006,2012, 2012.
APA	鄢庆枇,陈强,邹文政,马甡,庄峙厦,&王小如.(2012).不同环境条件对溶藻弧菌粘附大黄鱼肠粘液的影响.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sckx200602018&dbcode=CJFQ&dbname=CJFQ2006.
MLA	鄢庆枇,et al."不同环境条件对溶藻弧菌粘附大黄鱼肠粘液的影响".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sckx200602018&dbcode=CJFQ&dbname=CJFQ2006 (2012).