红毛藻中多组分藻胆蛋白及其亚基的分离

CORC > 集美大学

	红毛藻中多组分藻胆蛋白及其亚基的分离
	郑江 ; 高亚辉 ; 王文星 ; 黄水英
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zsck200304003&dbcode=CJFQ&dbname=CJFQ2003
	2012-06-05 ; 2012-06-05
关键词	红毛藻藻胆蛋白亚基分离 Bangia fusco-purpurea phycobiliprotein subunit separation Q946
其他题名	Separation of mutiple-phycobiliproteins and its subunits from Bangia fusco-purpurea
中文摘要	采用DEAE-52离子交换柱对红毛藻(Bangiafusco purpureaLyngb.)中的藻胆蛋白进行分离,依次洗脱出蓝色、紫色、深红色、红色和浅红色等几种组分的藻胆蛋白;经光谱分析发现蓝色组分为R-藻蓝蛋白,紫色组分为1种三峰型的藻胆蛋白,深红色组分和红色组分为2种不同但又非常相似的藻红蛋白,其中深红色组分可能与某些色素物质结合较为紧密,而最后洗脱出的浅红色组分则为变性的藻红蛋白。尿素洗脱结果表明,蓝色组分的R-藻蓝蛋白是由2种双峰型的亚基构成。本研究旨为揭示藻胆蛋白的能量传递机制提供理论和技术依据。; The dry sample of Bangia fuscopurpurea WTLyngb was collected from a cultural farm in Fujian. By means of DEAE-52 column chromatography, the blue, purple, dark red, red and light red components of phycobiliprotein were eluted from the B. fuscopurpurea in proper order. By absorption spectrum analysis, the results show that the blue component is R-phycobiliprotein(R-PC); the purple component is a tripeak phycobiliprotein; the dark red and red components are two kinds of resemblance phcoerythrins while the dark red component can be closely united with some pigments and the light red component, which is eluted at the last, is the denaturalized phycoerythrin. By analysis on the urea elution result, the blue R-PC is composed of two kinds of doublepeak subunits. The purpose of this study is to understand the mechanism of energy transportation in phycobiliprotein.; 【作者单位】厦门大学生命科学学院；集美大学生物工程学院；集美大学生物工程学院福建厦门　361005；集美大学水产学院；福建厦门　361021；福建厦门　361005；福建厦门　361021；福建厦门　361021；【作者英文名】ZHANG Jiang1,2, GAO Yahui1, WANG Wenxing3, HUANG Suiying3(1. School of Life Sciences, Xiamen University, Xiamen 361005, China; 2. School of Fisheries, Jimei University,Xiamen 361021, China; 3. School of Bioengineering, Jimei Uni
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12474]
专题	集美大学
推荐引用方式 GB/T 7714	郑江,高亚辉,王文星,等. 红毛藻中多组分藻胆蛋白及其亚基的分离[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zsck200304003&dbcode=CJFQ&dbname=CJFQ2003,2012, 2012.
APA	郑江,高亚辉,王文星,&黄水英.(2012).红毛藻中多组分藻胆蛋白及其亚基的分离.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zsck200304003&dbcode=CJFQ&dbname=CJFQ2003.
MLA	郑江,et al."红毛藻中多组分藻胆蛋白及其亚基的分离".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zsck200304003&dbcode=CJFQ&dbname=CJFQ2003 (2012).