红毛藻藻红蛋白的粗提方法比较及不同光照条件下藻胆蛋白变性机制的初步探讨

CORC > 集美大学

	红毛藻藻红蛋白的粗提方法比较及不同光照条件下藻胆蛋白变性机制的初步探讨
	郑江 ; 高亚辉 ; 王文星 ; 黄水英
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=xdzk200301026&dbcode=CJFQ&dbname=CJFQ2003
	2012-06-05 ; 2012-06-05
关键词	红毛藻藻红蛋白提取照射变性 Bangia fusco-pururea phycoerythin extraction irradiation denaturalization Q946
其他题名	Studies on Crude Extraction Methods of Phycoerythrin from Bangia fusco-purpurea and Denaturalization Mechanism of Phycobiliprotein in Different Irradiation Conditions
中文摘要	比较研究了多种细胞破碎方法和盐析方法对红毛藻中藻红蛋白的提取效果,确定组织捣碎法为效率较高的细胞破碎方法,用饱和度为60%的硫酸铵按"改进结晶法"进行盐析为较佳的盐析方法.探讨了冻融法的作用机制.同时对藻红蛋白盐析液在不同照射条件下的变性机制进行了初步的研究和探讨,研究显示光照有可能使藻胆蛋白的摩尔消光系数发生变化.; Different methods of cell fragmentation and salting out were compared in the present study to obtain the optimal method for extraction of phycoerythin from the redalga Bangia Fuscopururea. The results showed that tissuetrituration method was a relatively appropriate method for cell fragmentation compared with other methods, and the improved crystallization method with 60% saturation degree of ammonium sulfate was a more efficient salting out method than any other method used. The mechanism of freezing and thawing method was also discussed. The possible denaturalization mechanism of phycobiliprotein in salting out solution under different kinds of irradiation was experimentally studied. It was suggested that the irradiation could change the molar extinction coefficients of phycobiliprotein and caused the denaturalization of phycobiliprotein.; 【作者单位】厦门大学生命科学学院；集美大学生物工程学院；集美大学生物工程学院福建厦门361005；集美大学水产学院；福建厦门361005；福建厦门361021；福建厦门361021；【作者英文名】ZHENG Jiang1,2, GAO Yahui1, WANG Wenxing3, HUANG Suiying3(1. School of Life Sciences, Xiamen University, Xiamen 361005, China;2. School of Fisheries, Jimei University, 3. School of Bioengineering, Jimei University, Xiamen 3610
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12466]
专题	集美大学
推荐引用方式 GB/T 7714	郑江,高亚辉,王文星,等. 红毛藻藻红蛋白的粗提方法比较及不同光照条件下藻胆蛋白变性机制的初步探讨[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=xdzk200301026&dbcode=CJFQ&dbname=CJFQ2003,2012, 2012.
APA	郑江,高亚辉,王文星,&黄水英.(2012).红毛藻藻红蛋白的粗提方法比较及不同光照条件下藻胆蛋白变性机制的初步探讨.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=xdzk200301026&dbcode=CJFQ&dbname=CJFQ2003.
MLA	郑江,et al."红毛藻藻红蛋白的粗提方法比较及不同光照条件下藻胆蛋白变性机制的初步探讨".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=xdzk200301026&dbcode=CJFQ&dbname=CJFQ2003 (2012).