CORC  > 集美大学
福建官井洋大黄鱼AFLP指纹多态性的研究
王志勇 ; 王艺磊 ; 林利民 ; 邱淑贞 ; 本信明
刊名http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zsck200203002&dbcode=CJFQ&dbname=CJFQ2002
2012-06-05 ; 2012-06-05
关键词大黄鱼 野生种群 养殖群体 AFLP 多态性 Pseudosciaena crocea wild population cultured population AFLP polymorphism Q959.4
其他题名Genetic polymorphisms in wild and cultured large yellowcroaker Pseudosciaena crocea using AFLP fingerprinting </td></tr> <tr><td class="col-xs-1 right"><strong>中文摘要</strong></td><td class="col-xs-9 table-display-inline">对福建官井洋大黄鱼(Pseudosciaenacrocea)野生种群和2个养殖群体进行5对选择性引物(共37个标本)的AFLP分析。结果表明,共检出503个不同的扩增片段(50~450bp),每对引物扩增出的片段数目为76~155个;在5对引物检出的扩增片段中,101个(20.1%)为全部37个受试个体共有,312个(62.0%)为部分野生与养殖个体共有,53个(10.5%)仅见于野生个体,37个(7.4%)仅见于养殖个体,养殖群体中出现野生种群所没有的扩增片段;野生种群、养殖1、养殖2多态片段比例分别为76.6%、70.6%、69.2%,遗传差异度分别为0.2464、0.2322、0.2299,养殖群体多态片段比例与个体间的遗传差异度均低于野生种群。养殖群体的遗传多样性水平较低,遗传变异相对贫乏。; <Abstrcat> The wild individuals of Pseudosciaena crocea were collected from Guanjing Sea area, East China Sea. The cultured individuals were collected from two cultured populations in two different culture farms in Fujian Province, which were artificially breeding F5-F7. Totally 37 individuals were used. Five primer pairs (E-AAC/M-CTC, E-AAC/M-CAA, E-ACG/MCAG, E-ACG/M-CTT, E-AGG/M-CTG) were used and 503 selectively amplified fragments were detected, ranging from 50 to 450 bp in size, and the number of fragments produced by each pair of primers were 76-155. Among the 503 fragments, 101 bands presented in the total 37 individuals, and 312 bands only presented in part of the wild fish and the cultured, 37 only in the cultured, 53 only in the wild. In the cultured individuals, some fragments presented were not found in the wild individuals. The mean percentages of polymorphic bands of the three populations varied from 69.2% to 76.6% and the mean genetic differences within populations varied from 0.229 9 to 0.246 4, and the maximums all presented in the wild population, indicating the cultured populations had lower polymorphisms. The average genetic distance between the wild and the cultured populations is larger than that between the two cultured populations.; 【作者单位】集美大学水产学院; 集美大学水产学院; 东京水产大学资源育成学科; 东京水产大学资源育成学科 福建厦门361021; 福建厦门361021; 日本东京108-8477; 日本东京108-8477;【作者英文名】WANG Zhiyong1,WANG Yilei1,LIN Limin1,KHOO Sock Kean2,OKAMOTO Nobuaki2(1. Fisheries College, Jimei University, Xiamen 361021, China;2. Department of Aquatic Biosciences, Tokyo University of Fisheries, Konan 4-5-7, MinatoKu, Tokyo</td></tr> <tr><td class="col-xs-1 right"><strong>语种</strong></td><td class="col-xs-9 table-display-inline">中文 </td></tr> <tr><td class="col-xs-1 right"><strong>内容类型</strong></td><td class="col-xs-9 table-display-inline"><a class="red-decoration red-border" href="/simple-search?query1=%22%E6%9C%9F%E5%88%8A%E8%AE%BA%E6%96%87%22&field1=dc.type&advanced=false">期刊论文</a> </td></tr> <tr><td class="col-xs-1 right"><strong>源URL</strong></td><td class="col-xs-9 table-display-inline" style="word-break:break-all;">[<a target='_blank' href="http://ir.calis.edu.cn/hdl/235041/12206">http://ir.calis.edu.cn/hdl/235041/12206</a>]  <a href="#" class="itemb" title="无法访问全文,请点击条目查看详细资料"><img src="/themes/default/image/downing1.png" style="width:15px;" border="0"></a></td></tr> <tr><td class="col-xs-1 right"><strong>专题</strong></td><td class="col-xs-9 table-display-inline"><a target='_blank' href="/handle/1471x/1198506">集美大学</a><br/></td></tr> <tr><td class="col-xs-1 right"><strong>推荐引用方式</strong><br><strong>GB/T 7714</strong></td><td class="col-xs-9 table-display-inline"> 王志勇,王艺磊,林利民,等. 福建官井洋大黄鱼AFLP指纹多态性的研究[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zsck200203002&dbcode=CJFQ&dbname=CJFQ2002,2012, 2012. </td></tr> <tr><td class="col-xs-1 right"><strong>APA</strong></td><td class="col-xs-9 table-display-inline"> 王志勇,王艺磊,林利民,邱淑贞,&本信明.(2012).福建官井洋大黄鱼AFLP指纹多态性的研究.<i>http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zsck200203002&dbcode=CJFQ&dbname=CJFQ2002</i>. </td></tr> <tr><td class="col-xs-1 right"><strong>MLA</strong></td><td class="col-xs-9 table-display-inline"> 王志勇,et al."福建官井洋大黄鱼AFLP指纹多态性的研究".<i>http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=zsck200203002&dbcode=CJFQ&dbname=CJFQ2002</i> (2012). </td></tr> </table> <table class="title-entry-fixed" cellspacing="0" cellpadding="0" border="0"><tr><td class="border-bottom-red"><strong><a class="red-font-15">个性服务</a></strong></td></tr><tr><td><a class="icon-padding-l icon-view" href="/usage-stats?stats_object=item&object_id=1292242">查看访问统计</a></td></tr><tr><td class="border-bottom-red"><strong><a class="red-font-15">相关权益政策</a></strong></td></tr><tr><td><a class="null-value">暂无数据</a></td></tr><tr><td class="border-bottom-red"><strong><a class="red-font-15">收藏/分享</a></strong></td></tr><tr><td><div class="social-share"></div></td></tr></table></td></tr></table> <div id="loopedSlider"> </div> <table width="33%" border="0" align="center" cellpadding="3" cellspacing="3"> <tr> <td> <form method="get" action="/handle/1471x/1316098" id="viewItemForm"> <input type="text" name="workspace_id" value="" style="display: none;" /> <!--Modified by lijw,7/27/2014.--> <!--<input type="submit" name="submitThis" value="简要信息" />--> <input type="text" name="mode" value="simple" style="display: none;"/> <input type="text" name="page" id="commentCurrPage" value="0" style="display: none;"/> <input type="text" name="rndComment" id="rndComment" value="0" style="display: none;"/> </form> </td> </tr> </table> <div class="comment"> <div style=" border:1px solid #ccc; 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$("#cscd-citation-data").html("被引频次[CSCD]:"+data.citation_count); }*/ else { $("#cscd-citation-data").html("无"); $("#cscd-citation-data").css("color","#999"); } }, "html") } $(".pager").pager({ lang: true, pagenumber: currentPage, pagecount: pageCount, buttonClickCallback: PageClick }); $(".class-view-swf").click(function() { var id=this.id.substring(this.id.lastIndexOf("-")+1); if(id!="") { /*--var bitHandle=document.getElementById("span-view-swf-"+id); if(bitHandle!=null && bitHandle.innerHTML!="") { var swf=baseURL+"/get-swf?bitHandle="+bitHandle.innerHTML; displaySWF(swf); } else*/ { var imgLoading=document.getElementById("id-view-swf-loading-"+id); imgLoading.style.display = ""; $.get(""+"/bitstream", { orignalBitID:id, itemID:1292242, method:"get-swf-bit", timeid:new Date().getTime() }, function(data){ imgLoading.style.display = "none"; if(data.substring(0,2)=="ok") { data=data.substring(3); displaySWF(baseURL+"/get-swf?bitHandle="+data); } else alert("暂不允许浏览"); }, "html") } } }); $(".class-view-video").click(function() { var id=this.id.substring(this.id.lastIndexOf("-")+1); if(id!="") { var imgHandle=document.getElementById("span-view-video-img-"+id).innerHTML; var videoHandle=document.getElementById("span-view-video-"+id).innerHTML; document.getElementById("div-video-title").innerHTML = document.getElementById("span-bit-name-"+id).innerHTML; jwplayer("myElement").setup({ width:620, height:480, file:videoHandle, image: imgHandle, primary: "flash" }); } }); setTimeout(function () { var editorone=document.getElementById("editor1"); if(editorone!=null) CKEDITOR.replace('editor1'); //CKEDITOR.replace('editor2'); //CKEDITOR.replace('editor3'); CKEDITOR.replace("editor4"); },500); $("#div_modify_comment").on('show.bs.modal', function(){ var $this = $(this); var $modal_dialog = $this.find('.modal-dialog'); $this.css('display', 'block'); $modal_dialog.css({'margin-top': Math.max(0, ($(window).height() - $modal_dialog.height()) / 2) }); }); }); /* function commitForm(action,obj) { $("#callURL").attr("value",window.location.href); if(action=="add") { var editor_data = CKEDITOR.instances.editor1.getData(); //var editor_data = $("#editor1").attr("value"); if(editor_data=="") { alert("请输入评论内容"); } else submitForm('add',editor_data,'0'); } else if(action=="del") { if (confirm('您确定要删除吗?')) { var id=obj.id.replace("delete_","") submitForm('delete','',id); } } }*/ function displaySWF() { var loopedSlider=document.getElementById("loopedSlider"); var viewerPlaceHolders=getElementsByClass(loopedSlider, "viewerPlaceHolder", "div"); if(viewerPlaceHolders!=null) { for(var i=0; i<viewerPlaceHolders.length; i++) { var viewerPlaceHolder = viewerPlaceHolders[i]; var swf = viewerPlaceHolder.id; //viewerPlaceHolder.style.display="block"; viewerPlaceHolder.style.width="640px"; viewerPlaceHolder.style.height="750px"; var fp = new FlexPaperViewer( ""+'/FlexPaper_1.5.1_flash/FlexPaperViewer', viewerPlaceHolder, { config : { SwfFile : swf, Scale : 0.6, ZoomTransition : 'easeOut', ZoomTime : 0.5, ZoomInterval : 0.2, FitPageOnLoad : true, FitWidthOnLoad : true, FullScreenAsMaxWindow : false, ProgressiveLoading : false, MinZoomSize : 0.2, MaxZoomSize : 5, SearchMatchAll : false, InitViewMode : 'Portrait', PrintPaperAsBitmap : false, ViewModeToolsVisible : true, ZoomToolsVisible : true, NavToolsVisible : true, CursorToolsVisible : true, SearchToolsVisible : true, localeChain: 'en_US' }}); } } } function commitCommentForm(action,obj) { if(action=="add") { var editor_data = CKEDITOR.instances.editor1.getData(); if(editor_data=="") { alert("请输入评论内容"); } else { submitAlert('loading','正在提交...'); $("#commentCurrPage").attr("value",1); $.post(baseURL+"/comment", { "type":"comment", "content":editor_data, "action":action, "resourceID":'1292242', "num":Math.random() }, function(data){ if(data.substring(0,2)=="ok") submitAlert('succeed',data.substring(3)); else submitAlert('error',data); setTimeout("refreshThis();",1000); $("#item-comment").hide(); }, "html") } } else if(action=="del") { if (confirm('您确定要删除吗?')) { var comment_id=obj.id.replace("delete_","") //submitForm('delete','',id); submitAlert('loading','正在删除...'); $("#commentCurrPage").attr("value",1); $.post(baseURL+"/comment", { "type":"comment", "commentID":comment_id, "action":action, "num":Math.random() }, function(data){ if(data.substring(0,2)=="ok") submitAlert('succeed',data.substring(3)); else submitAlert('error',data); setTimeout("refreshThis();",1000); }, "html") } } } function commitObjectionForm() { var value_editor_data = $("#editor2").val(); // CKEDITOR.instances.editor2.getData(); var value_input_title; var value_email; var value_organization; var value_code; if(value_editor_data.trim()=="") { alert("请输入异议内容"); return; } var logined=false; if(!logined) { var input_email = document.getElementById("input-email-objection"); value_email=input_email.value.trim(); if(value_email=="") { alert("请输入Email"); return; } var input_code = document.getElementById("input-code-objection"); value_code=input_code.value; if(value_code=="") { alert("请输入验证码"); return; } var input_organization = document.getElementById("input-email-organization"); value_organization=input_organization.value.trim(); } submitAlert('loading','正在提交...'); $.post(baseURL+"/comment", { "type":"objection", "content":value_editor_data, "resourceID":'1292242', "organization":value_organization, "email":value_email, "code":value_code, "num":Math.random() }, function(data) { if(dialog!=null) dialog.close(); if(data.length>3 && data.substring(0,2)=="ok") { data=data.substring(3); submitAlert('succeed',data); document.getElementById("commentFormObjection").reset(); $("#item-comment").hide(); // CKEDITOR.instances.editor2.setData(""); $("#editor2").val(""); getCaptcha(baseURL, "objection"); //setTimeout("resetForm('commentFormObjection');",3000); } else { submitAlert('error',data); } //setTimeout("refreshThis();",1000); }, "html") } function commitFeedbackForm() { var value_editor_data = $("#editor3").val();// CKEDITOR.instances.editor3.getData(); var value_input_title; var value_email; var value_code; var input_title = document.getElementById("input-title-feedback"); value_input_title = input_title.value.trim(); if(value_input_title=="") { alert("请填写标题"); return; } if(value_editor_data.trim()=="") { alert("请输入反馈内容"); return; } var logined=false; if(!logined) { var input_email = document.getElementById("input-email-feedback"); value_email=input_email.value.trim(); value_code=document.getElementById("input-code-objection").value; var input_code = document.getElementById("input-code-feedback"); value_code=input_code.value; if(value_code=="") { alert("请输入验证码"); return; } } submitAlert('loading','正在提交...'); $.post(baseURL+"/comment", { "type":"feedback", "content":value_editor_data, "resourceID":'1292242', "title":value_input_title, "email":value_email, "code":value_code, "num":Math.random() }, function(data){ if(dialog!=null) dialog.close(); if(data.length>3 && data.substring(0,2)=="ok") { data=data.substring(3); submitAlert('succeed',data); document.getElementById("commentFormFeedback").reset(); $("#item-comment").hide(); // CKEDITOR.instances.editor3.setData(""); $("#editor3").val(""); getCaptcha(baseURL, "feedback"); //setTimeout("resetForm('commentFormObjection');",3000); } else { submitAlert('error',data); } }, "html") } function submitForm(action, comment, comment_id) { submitAlert('loading','正在提交...'); $("#commentCurrPage").attr("value",1); $.post(baseURL+"/comment", { "comment":comment, "action":action, "resourceID":'1292242', "commentID":comment_id }, function(data){ submitAlert('succeed',data); setTimeout("refreshThis();",1000); }, "html") } function refreshThis() { $("#commentCurrPage").attr("value",1); $("#rndComment").attr("value",new Date().getTime()); $("#viewItemForm").submit(); } var dialog; function submitAlert(iconName, text) { dialog=art.dialog({ title:'提示', drag: false, resize: false, lock: true, background: '#ccc', // 背景色 opacity: 0.57, // 透明度 content: text, icon: iconName, ok:true }); } function resetForm(formName) { if(dialog!=null) dialog.close(); document.getElementById(formName).reset(); if(formName=="") $("#editor2").val(""); // CKEDITOR.instances.editor2.setData(""); } function alertLogin() { dialog=art.dialog({ title:'提示', drag: false, resize: false, lock: true, background: '#ccc', // 背景色 opacity: 0.57, // 透明度 content: '请您先登录!', icon: 'warning', ok:function () { window.location.href=baseURL+"/password-login"; return true; }, cancelVal: '取消', cancel: true }); } PageClick = function(pageclickednumber) { $(".pager").pager({ lang: true, pagenumber: pageclickednumber, pagecount: pageCount, buttonClickCallback: PageClick }); $("#commentCurrPage").attr("value",pageclickednumber); $("#rndComment").attr("value",new Date().getTime()); $("#viewItemForm").submit(); } function newReplay() { //var logined='false'; //if(logined=="true") //{ $("#item-comment").show(); window.location.href=window.location.href+"#new_comment"; //} //else //{ // alertLogin(); //} } function showDetails(id) { id="div_details_"+id; dialog=art.dialog({ title:'详细信息', drag: false, width:800, resize: false, lock: true, background: '#ccc', // 背景色 opacity: 0.57, // 透明度 content: $("#"+id).html(), }); } function submitComment () { var comment_id = $("#comment_id").val(); var editor_data = CKEDITOR.instances.editor4.getData(); if(editor_data==="") { alert("请输入评论内容"); return; } $.post(baseURL+"/comment", { "type":"comment", "commentID":comment_id, "action":"modify", "content":editor_data, "num":Math.random() }, function(data){ if(data.substring(0,2)=="ok") submitAlert('succeed',data.substring(3)); else submitAlert('error',data); CKEDITOR.instances.editor4.setData(""); setTimeout("refreshThis();",1000); }, "html"); } function modifyComment(obj) { var comment_id=obj.id.replace("modify_","") $("#comment_id").val(comment_id); CKEDITOR.instances.editor4.setData($("#comment_"+comment_id).val()); $("#div_modify_comment").modal(); } $(function(){ $(".table.detailed-table").parent().addClass("tabel-body"); }); </script> <style type="text/css"> .details { display:none; } .jiathis_style .ico{ float: left; font-size: 12px; text-decoration: none; line-height: 18px!important; text-align: left; overflow: hidden; display: block!important; height: 16px!important; line-height: 16px!important; padding-left: 20px!important; cursor: pointer; } .jiathis_style .citeULike{ background: url(/image/bookmarks/citeulike.gif) no-repeat left; } </style> </div> </div> <div class="row4"> <div class="container-1170"> <div id="footer" class="clear"> <div class="footbox"> <ul class="col-md-3"> <li><h2>相关链接</h2></li> <li><a href="http://www.openaire.eu">欧盟学术资源开放存取平台</a></li> <li><a href="http://ir.calis.edu.cn">CALIS高校机构知识库</a></li> <li><a href="http://tair.org.tw">台湾学术机构典藏</a></li> <li><a href="http://hkir.ust.hk/hkir">香港机构知识库整合系统</a></li> <li><a href="http://www.irgrid.ac.cn">中国科学院机构知识库网格系统</a></li> </ul> <div class="pull-right col-md-6 col-md-offset-3"> <ul class="col-md-3 col-md-offset-4"> <li><h2>CORC</h2></li> <li><a href="#">关于我们</a></li> <li><a href="#">服务条款</a></li> <li><a href="#">隐私条款</a></li> </ul> <ul class="cell-color col-md-5"> <li><h2>联系我们</h2></li> <li><a href="#"><i class="fa fa-phone-square"></i>0931-8270076</a></li> <li><a href="#"><i class="fa fa-envelope"></i>cspace@llas.ac.cn</a></li> <li><a href="#"><i class="fa fa-weixin"></i>China-OR</a></li> </ul> <ul class="list-inline list-inline col-md-8 col-md-offset-4"> <li class="col-md-3"><a class="bg-china" href="">中文简体</a></li> <li class="col-md-2 col-md-offset-1"><i class="fa fa-2x fa-weixin"></i></li> <li class="col-md-2"><i class="fa fa-2x fa-weibo"></i></li> </ul> </div> </div> <div style="clear: both;"></div> </div> </div> <hr class="row4-bottom"> <div style="height: 30px;"> <div class="position-a"> <p>©版权所有 ©2017 CSpace - Powered by <a href="http://www.cspace.org.cn/" style='color:#666666'>CSpace</a></p> </div> </div> </div> </body> </html>