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	Comparative genetic analysis of Arabidopsis purple acid phosphatases AtPAP10, AtPAP12, and AtPAP26 provides new insights into their roles in plant adaptation to phosphate deprivation
	Liangsheng Wang ; Shan Lu ; Ye Zhang ; Zheng Li ; Xiaoqiu Du ; Dong Liu ; Liangsheng Wang ; Shan Lu ; Ye Zhang ; Zheng Li ; Xiaoqiu Du ; Dong Liu
	2016-03-30 ; 2016-03-30
关键词	Phosphate starvation responses acid phosphatase activity atpap mutants AtPAP overexpression tolerance to phosphate deprivation Q946
其他题名	Comparative genetic analysis of Arabidopsis purple acid phosphatases AtPAP10, AtPAP12, and AtPAP26 provides new insights into their roles in plant adaptation to phosphate deprivation
中文摘要	Induction and secretion of acid phosphatases(APases) is thought to be an adaptive mechanism that helps plants survive and grow under phosphate(Pi) deprivation. In Arabidopsis, there are 29 purple acid phosphatase(AtPAP)genes. To systematically investigate the roles of different AtPAPs, we first identified knockout or knock‐down T‐DNA lines for all 29 AtPAP genes. Using these atpap mutants combined with in‐gel and quantitative APase enzyme assays,we demonstrated that AtPAP12 and AtPAP26 are two major intracellular and secreted APases in Arabidopsis while AtPAP10is mainly a secreted APase. On Pi‐deficient(P) medium or Pmedium supplemented with the organophosphates ADP and fructose‐6‐phosphate(Fru‐6‐P), growth of atpap10 was significantly reduced whereas growth of atpap12 was only moderately reduced, and growth of atpap26 was nearly equal to that of the wild type(WT). Overexpression of the AtPAP12 or AtPAP26 gene, however, caused plants to grow better on Por P medium supplemented with ADP or Fru‐6‐P. Interestingly, Pi levels are essentially the same for the WT and overexpressing lines, although these two types of plants have significantly different growth phenotypes. These results suggest that the APases may have other roles besides enhancing internal Pi recycling or releasing Pi from external organophosphates for plant uptake.; Induction and secretion of acid phosphatases(APases) is thought to be an adaptive mechanism that helps plants survive and grow under phosphate(Pi) deprivation. In Arabidopsis, there are 29 purple acid phosphatase(AtPAP)genes. To systematically investigate the roles of different AtPAPs, we first identified knockout or knock‐down T‐DNA lines for all 29 AtPAP genes. Using these atpap mutants combined with in‐gel and quantitative APase enzyme assays,we demonstrated that AtPAP12 and AtPAP26 are two major intracellular and secreted APases in Arabidopsis while AtPAP10is mainly a secreted APase. On Pi‐deficient(P) medium or Pmedium supplemented with the organophosphates ADP and fructose‐6‐phosphate(Fru‐6‐P), growth of atpap10 was significantly reduced whereas growth of atpap12 was only moderately reduced, and growth of atpap26 was nearly equal to that of the wild type(WT). Overexpression of the AtPAP12 or AtPAP26 gene, however, caused plants to grow better on Por P medium supplemented with ADP or Fru‐6‐P. Interestingly, Pi levels are essentially the same for the WT and overexpressing lines, although these two types of plants have significantly different growth phenotypes. These results suggest that the APases may have other roles besides enhancing internal Pi recycling or releasing Pi from external organophosphates for plant uptake.
语种	英语 ; 英语
内容类型	期刊论文
源URL	[http://ir.lib.tsinghua.edu.cn/ir/item.do?handle=123456789/148408]
专题	清华大学
推荐引用方式 GB/T 7714	Liangsheng Wang,Shan Lu,Ye Zhang,et al. Comparative genetic analysis of Arabidopsis purple acid phosphatases AtPAP10, AtPAP12, and AtPAP26 provides new insights into their roles in plant adaptation to phosphate deprivation[J],2016, 2016.
APA	Liangsheng Wang.,Shan Lu.,Ye Zhang.,Zheng Li.,Xiaoqiu Du.,...&Dong Liu.(2016).Comparative genetic analysis of Arabidopsis purple acid phosphatases AtPAP10, AtPAP12, and AtPAP26 provides new insights into their roles in plant adaptation to phosphate deprivation..
MLA	Liangsheng Wang,et al."Comparative genetic analysis of Arabidopsis purple acid phosphatases AtPAP10, AtPAP12, and AtPAP26 provides new insights into their roles in plant adaptation to phosphate deprivation".(2016).

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