| HIV-1假病毒包装方法的探讨 | |
| 徐树莹 ; 乔录新 ; 史宣玲 ; 徐萌 ; 魏飞力 ; 袁霖 ; 石英 ; 陈德喜 ; XU Shuying ; QIAO Luxin ; SHI Xuanling ; XU Meng ; WEI Feili ; YUAN Lin ; SHI Ying ; CHEN Dexi | |
| 2016-03-30 ; 2016-03-30 | |
| 关键词 | 人类免疫缺陷病毒Ⅰ型 假病毒 包装方法 Human immunodeficiency virus type Ⅰ Pseudotyped virus Packaging method R512.91 |
| 其他题名 | Optimization of packaging the HIV-1 pseudotyped virus |
| 中文摘要 | 目的通过不同方案包装HIV-1假病毒,确定获得高重复性、高稳定性和高滴度假病毒的方法。方法从转染效率影响因素出发,通过不同的转染方案,利用pNL4-3 LucR-E-质粒与VSV-G质粒共转染293细胞包装病毒,分别于48 h和72 h收取假病毒并重新感染293细胞,48 h后测荧光素酶报告基因。结果成功包装出HIV-1假病毒,不同包装方案的病毒滴度不同,测得报告基因最高接近107 RLUs。结论应用PEI转染试剂、大提质粒pNL4-3︰VSV-G为4︰1时,转染效率最高,包装的假病毒滴度最高。; Objective To package HIV-1 pseudotyped virus through different ways, to define methodology of packaging high reproductive, high stable and high titer pseudotyped virus. Methods In view of the transfection efficiency factors, through different transfection ways, pNL4-3 LucR-E- plasmid and VSV-G plasmid were transfected into 293 cells packaging virus, and the HIV-1 pseudotyped virus after 48 h and 72 h were collected, respectively, then reporter gene after infecting 293 cells for 48 h were detected. Results HIV-1 pseudotyped virus were packaged successfully, the virus titer differs from packaging ways, and reporter gene measured up to 107 RLUs. Conclusion When using PEI transfection reagent, and plasmid pNL4-3 ︰ VSV-G as 4 ︰ 1, the highest transfection efficiency and the highest viral titer could received. |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://ir.lib.tsinghua.edu.cn/ir/item.do?handle=123456789/147376]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 徐树莹,乔录新,史宣玲,等. HIV-1假病毒包装方法的探讨[J],2016, 2016. |
| APA | 徐树莹.,乔录新.,史宣玲.,徐萌.,魏飞力.,...&CHEN Dexi.(2016).HIV-1假病毒包装方法的探讨.. |
| MLA | 徐树莹,et al."HIV-1假病毒包装方法的探讨".(2016). |
| 个性服务 |
| 查看访问统计 |
| 相关权益政策 |
| 暂无数据 |
| 收藏/分享 |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论