| HPLC/TOF-MS和HPLC/IT-MS~n联合用于补骨脂药材的化学成分分析 | |
| 罗娟敏 ; 肖雪 ; 洪流 ; 田耀伟 ; 郭磊 ; 苏加坤 ; 蔡继宝 ; 罗国安 ; LUO Juan-min ; XIAO Xue ; HONG Liu ; TIAN Yao-wei ; GUO Lei ; SU Jia-kun ; CAI Ji-bao ; LUO Guo-an | |
| 2016-03-30 ; 2016-03-30 | |
| 关键词 | 补骨脂药材 高效液相色谱 飞行时间质谱 离子阱多级质谱 多成分分析 补骨脂素 异补骨脂素 Psoraleae Fructus HPLC TOF-MS IT-MSn multi-component analysis psoralen isopsoralen R284.1 |
| 其他题名 | Analysis on chemical constitutes in Psoraleae Fructus by combination of HPLC/ TOF-MS and HPLC/IT-MS~n |
| 中文摘要 | 目的建立一种结合高效液相色谱飞行时间质谱(HPLC/TOF-MS)及高效液相色谱离子阱多级质谱(HPLC/IT-MSn)对补骨脂药材进行化学成分定性分析的方法。方法 HPLC色谱分离采用Phenomenex luna C18(5μm)反相色谱柱为分析柱,0.1%甲酸水溶液-乙腈为流动相,梯度洗脱,检测波长为254 nm;质谱定性采用飞行时间质谱结合离子阱多级质谱,正负离子模式扫描。结果在优化的液质联用条件下,飞行时间质谱筛查出13种成分的分子式,采用离子阱多级质谱鉴定分析此13种成分。结论 HPLC/TOF-MS和HPLC/IT-MSn联合的分析方法,为中药的多成分分析提供了一种有效、可靠的新模式,此模式也同样适用于其他复杂体系的分析。; Objective To establsh a combinative HPLC/TOF-MS and HPLC/IT-MSn method for quantitative analysis on chemical constituents in Psoraleae Fructus. Methods Separation was performed on a Phenomenex Luna C18(5 μm) reverse-phase column. The mobile phase consisted of water containing 0.1% formic acid and acetonitrile was used as gradient elute. The flow rate was 1 mL/min. A combination of time-of-flight mass spectrometer(TOF-MS) and ion trap tandem mass spectrometer(IT-MSn) was applied for qualitative analysis under positive and negative ion mode. Results Under optimized LC/MS condition, the molecular formulae of 13 components were screened by TOF-MS and all of them were then identified by IT-MSn. Conclusion The established combinative HPLC/MS method could thus be considered as an effective and credible pattern for the analysis on the multi-components of Chinese materia medica and it also could be used for as the analysis on other complex systems. |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://ir.lib.tsinghua.edu.cn/ir/item.do?handle=123456789/145915]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 罗娟敏,肖雪,洪流,等. HPLC/TOF-MS和HPLC/IT-MS~n联合用于补骨脂药材的化学成分分析[J],2016, 2016. |
| APA | 罗娟敏.,肖雪.,洪流.,田耀伟.,郭磊.,...&LUO Guo-an.(2016).HPLC/TOF-MS和HPLC/IT-MS~n联合用于补骨脂药材的化学成分分析.. |
| MLA | 罗娟敏,et al."HPLC/TOF-MS和HPLC/IT-MS~n联合用于补骨脂药材的化学成分分析".(2016). |
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