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	Determination of gouty arthritis' biomarkers in human urine using reversed-phase high-performance liquid chromatography
	Lei-Wen Xiang ; Jing Li ; Jin-Ming Lin ; Hai-Fang Li ; Lei-Wen Xiang ; Jing Li ; Jin-Ming Lin ; Hai-Fang Li
	2016-03-30 ; 2016-03-30
关键词	Gouty arthritis Creatinine Uric acid Hypoxanthine Xanthine High-performance liquid chromatography R589.7
其他题名	Determination of gouty arthritis' biomarkers in human urine using reversed-phase high-performance liquid chromatography
中文摘要	Creatinine, uric acid, hypoxanthine and xanthine are important diagnostic biomarkers in human urine for gouty arthritis or renal disease diacrisis. A simple method for simultaneous determination of these biomarkers in urine based on reversed-phase high-performance liquid chromatography(RPHPLC) with ultraviolet(UV) detector was proposed. After pretreatment by dilution, centrifugation and filtration, the biomarkers in urine samples were separated by ODS-BP column by elution with methanol/50 mM NaH2PO4 buffer solution at pH 5.26(5:95). Good linearity between peak areas and concentrations of standards was obtained for the biomarkers with correlation coefficients in the range of 0.9957–0.9993.The proposed analytical method has satisfactory repeatability(the recovery of data in a range of creatinine, uric acid, hypoxanthine and xanthine was 93.49–97.90%, 95.38–96.45%, 112.46–115.78% and90.82–97.13% with standard deviation of o5%, respectively) and the limits of detection(LODs, S/NZ3)for creatinine, uric acid, hypoxanthine, and xanthine were 0.010, 0.025, 0.050 and 0.025 mg/L,respectively. The established method was proved to be simple, accurate, sensitive and reliable for the quantitation of gouty arthritis' biomarkers in human urine samples. The ratio of creatinine to uric acid was found to be a possible factor for assessment of gouty arthritis.; Creatinine, uric acid, hypoxanthine and xanthine are important diagnostic biomarkers in human urine for gouty arthritis or renal disease diacrisis. A simple method for simultaneous determination of these biomarkers in urine based on reversed-phase high-performance liquid chromatography(RPHPLC) with ultraviolet(UV) detector was proposed. After pretreatment by dilution, centrifugation and filtration, the biomarkers in urine samples were separated by ODS-BP column by elution with methanol/50 mM NaH2PO4 buffer solution at pH 5.26(5:95). Good linearity between peak areas and concentrations of standards was obtained for the biomarkers with correlation coefficients in the range of 0.9957–0.9993.The proposed analytical method has satisfactory repeatability(the recovery of data in a range of creatinine, uric acid, hypoxanthine and xanthine was 93.49–97.90%, 95.38–96.45%, 112.46–115.78% and90.82–97.13% with standard deviation of o5%, respectively) and the limits of detection(LODs, S/NZ3)for creatinine, uric acid, hypoxanthine, and xanthine were 0.010, 0.025, 0.050 and 0.025 mg/L,respectively. The established method was proved to be simple, accurate, sensitive and reliable for the quantitation of gouty arthritis' biomarkers in human urine samples. The ratio of creatinine to uric acid was found to be a possible factor for assessment of gouty arthritis.
语种	英语 ; 英语
内容类型	期刊论文
源URL	[http://ir.lib.tsinghua.edu.cn/ir/item.do?handle=123456789/145897]
专题	清华大学
推荐引用方式 GB/T 7714	Lei-Wen Xiang,Jing Li,Jin-Ming Lin,et al. Determination of gouty arthritis' biomarkers in human urine using reversed-phase high-performance liquid chromatography[J],2016, 2016.
APA	Lei-Wen Xiang.,Jing Li.,Jin-Ming Lin.,Hai-Fang Li.,Lei-Wen Xiang.,...&Hai-Fang Li.(2016).Determination of gouty arthritis' biomarkers in human urine using reversed-phase high-performance liquid chromatography..
MLA	Lei-Wen Xiang,et al."Determination of gouty arthritis' biomarkers in human urine using reversed-phase high-performance liquid chromatography".(2016).

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