| 丹参脂溶性成分抑制体外血管新生及机制研究 | |
| 范雪梅 ; 任桂香 ; 梁琼麟 ; 王义明 ; 罗国安 ; FAN Xue-mei ; REN Gui-xiang ; LIANG Qiong-lin ; WANG Yi-ming ; LUO Guo-an | |
| 2016-03-30 ; 2016-03-30 | |
| 关键词 | 丹参脂溶性成分 血管新生 人脐静脉内皮细胞 实时荧光定量PCR 血管内皮生长因子 lipophilic components in Salvia miltiorrhiza angiogenesis human umbilical vein endothelial cells Real-time fluorescence quantification PCR vascular endothelial growth factor R285 |
| 其他题名 | Study on inhibitory effects and mechanism of lipophilic components in Salvia miltiorrhiza on angiogenesis in vitro |
| 中文摘要 | 该研究利用人脐静脉内皮细胞模型研究丹参脂溶性成分对血管新生的调节作用,并探讨其可能的作用机制。通过建立的细胞模型,确定丹参脂溶性成分对内皮细胞增殖活性和迁移能力的影响;随后利用实时荧光定量PCR技术检测药物作用后血管新生相关细胞因子VEGF-A,VEGF-C和MMP-9的基因表达变化。结果表明,5 mg·L~(-1)的丹参脂溶性成分抑制内皮细胞的增殖和迁移,同时VEGF-A,VEGF-C和MMP-9基因的表达被抑制。表明丹参脂溶性成分可能通过抑制VEGF-A和MMP-9基因的表达来抑制内皮细胞的增殖和迁移,从而达到抑制血管新生的作用。; In this study,the human umbilical vein endothelial cell model was used to study the regulating effect of lipophilic components in Salvia miltiorrhiza on angiogenesis,and explore its possible mechanism.The cell model was established to determine the effect of lipophilic components in S.miltiorrhiza on the proliferative activity and migration capacity of endothelial cells.Then the realtime fluorescence quantification PCR technology was applied to detect the changes in the gene expressions of angiogenesis-related cytokines VEGF-A,VEGF-C and MMP-9.The results showed that 5 mg稬~(-1) lipophilic components in 5.miltiorrhiza could inhibit the proliferation and migration of endothelial cells,and reduce the expression of VEGF-A and MMP-9 genes.It indicated that lipophilic components in S.miltiorrhiza may inhibit the proliferation and migration of endothelial cells by inhibiting the expression of VEGF-A and MMP-9 genes,so as to show the inhibitory effect on angiogenesis. |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://ir.lib.tsinghua.edu.cn/ir/item.do?handle=123456789/145894]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 范雪梅,任桂香,梁琼麟,等. 丹参脂溶性成分抑制体外血管新生及机制研究[J],2016, 2016. |
| APA | 范雪梅.,任桂香.,梁琼麟.,王义明.,罗国安.,...&LUO Guo-an.(2016).丹参脂溶性成分抑制体外血管新生及机制研究.. |
| MLA | 范雪梅,et al."丹参脂溶性成分抑制体外血管新生及机制研究".(2016). |
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