Quantifying the Effects of Contact Duration, Loading Rate, and Approach Velocity on P-Selectin-PSGL-1 Interactions Using AFM

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	Quantifying the Effects of Contact Duration, Loading Rate, and Approach Velocity on P-Selectin-PSGL-1 Interactions Using AFM
	Lv SQ(吕守芹); Ye ZY(叶志义); Zhu C(朱承); Long M(龙勉)
刊名	Polymer
	2006
通讯作者邮箱	cheng.zhu@me.gatech.edu ; mlong@imech.ac.cn
卷号	47 期号:7 页码:2539-2547
ISSN号	0032-3861
通讯作者	Zhu, C (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China.
中文摘要	Kinetics and its regulation by extrinsic physical factors govern selectin-ligand interactions that mediate tethering and rolling of circulating cells on the vessel wall under hemodynamic forces. While the force regulation of off-rate for dissociation of selectin-ligand bonds has been extensively studied, much less is known about how transport impacts the on-rate for association of these bonds and their stability. We used atomic force microscopy (AFM) to quantify how the contact duration, loading rate, and approach velocity affected kinetic rates and strength of bonds of P-selectin interacting with P-selectin glycoprotein ligand I (PSGL-1). We found a saturable relationship between the contact time and the rupture force, a biphasic relationship between the adhesion probability and the retraction velocity, a piece-wise linear relationship between the rupture force and the logarithm of the loading rate, and a threshold relationship between the approach velocity and the rupture force. These results provide new insights into how physical factors regulate receptor-ligand interactions.
学科主题	力学
类目[WOS]	Polymer Science
研究领域[WOS]	Polymer Science
关键词[WOS]	SELECTIN GLYCOPROTEIN LIGAND-1 ; CELL-ADHESION MOLECULES ; P-SELECTIN ; DYNAMIC STRENGTH ; BINDING-KINETICS ; IMMUNE-SYSTEM ; CATCH BONDS ; FORCE ; DISSOCIATION ; SHEAR
收录类别	SCI
语种	英语
WOS记录号	WOS:000236629900036
公开日期	2007-06-15 ; 2007-12-05 ; 2009-06-23
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/16643]
专题	力学研究所_力学所知识产出(1956-2008)
推荐引用方式 GB/T 7714	Lv SQ,Ye ZY,Zhu C,et al. Quantifying the Effects of Contact Duration, Loading Rate, and Approach Velocity on P-Selectin-PSGL-1 Interactions Using AFM[J]. Polymer,2006,47(7):2539-2547.
APA	吕守芹,叶志义,朱承,&龙勉.(2006).Quantifying the Effects of Contact Duration, Loading Rate, and Approach Velocity on P-Selectin-PSGL-1 Interactions Using AFM.Polymer,47(7),2539-2547.
MLA	吕守芹,et al."Quantifying the Effects of Contact Duration, Loading Rate, and Approach Velocity on P-Selectin-PSGL-1 Interactions Using AFM".Polymer 47.7(2006):2539-2547.