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	贴壁细胞电阻抗谱等效电路模型的建立与分析
	焦泉 ; 胥善治 ; 杨生胜 ; 秦晓刚 ; 王鹏 ; JIAO Quan ; XV Shan-zhi ; YANG Sheng-sheng ; QIN Xiao-gang ; WANG Peng
	2016-03-30 ; 2016-03-30
关键词	叉指电极 细胞电阻抗谱 等效电路模型 interdigitated electrodes electric cell-substrate impedance equivalent circuit model Q27
其他题名	Establishment and analysis of the equivalent circuit model of the electrical impedance of adherent cells
中文摘要	为了在生物电特性与生化特性之间建立明确的映射关系,采用微加工技术于硅玻璃基底上研制出了叉指结构金电极阵列,组装出了能实时、连续获得细胞电阻抗谱的测量装置。将子宫颈癌细胞接种于测量装置中,细胞在电极上进行正常贴附生长,使用Parstat4000连接测量装置获得了不同时刻频率范围为1 Hz~1 MHz的细胞电阻抗谱数据;在已有细胞阻抗谱等效电路模型基础上,根据实际测量阻抗谱数据,结合细胞生理过程电特性,提出了修正细胞阻抗模型。研究结果表明,该修正模型用于拟合阻抗数据的方差比原模型小,同时修正模型包含参数按变化趋势分作两组,分别反映细胞贴附生长过程中溶液特性的变化和细胞本身的变化,最终建立了细胞阻抗数据与细胞生理变化之间的定性关系。; In order to establish a clear relationship between the biological electrical characteristics and biochemical characteristics,an interdigitated gold electrode was designed and manufactured on a silica glass substrate by using micro-processing technology. Based on this substrate,a cell electrical measurement device that can measure continuous impedance spectroscopy in real-time was then fabricated. Cervical cancer cell was planted and then growing on the measurement cell. Impedance spectroscopy of the cell was then measured with Parstat4000,with frequency varying from 1 Hz to 1 MHz. Based on existed equivalent circuit model,a corrected model of interdigital electrodes was proposed. The result indicates that the variance of the corrected model is smaller than the original model. The parameters of corrected model can be devided into two sets which reflect the change of character of the solution and the cells while the cells were growing. Finally,a qualitative relationship between the impedance data and cell physiological change is build.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://ir.lib.tsinghua.edu.cn/ir/item.do?handle=123456789/144601]
专题	清华大学
推荐引用方式 GB/T 7714	焦泉,胥善治,杨生胜,等. 贴壁细胞电阻抗谱等效电路模型的建立与分析[J],2016, 2016.
APA	焦泉.,胥善治.,杨生胜.,秦晓刚.,王鹏.,...&WANG Peng.(2016).贴壁细胞电阻抗谱等效电路模型的建立与分析..
MLA	焦泉,et al."贴壁细胞电阻抗谱等效电路模型的建立与分析".(2016).

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