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	用分光光度计预测小球藻藻液浓度的研究
	潘竞洋 ; 赵之旭 ; 吴良柏 ; 张伟 ; 徐俊生 ; 陈玉桂 ; 付志德 ; PAN Jing-yang ; ZHAO Zhi-xu ; Gansu Endangered ; Animal Protection ; Centre of ; the State ; Forestry Administration
	2016-03-30 ; 2016-03-30
关键词	小球藻 最佳波段 当量浓度 magnetic monopole Dirac string gauge transformation Q949.211
其他题名	Concentration Prediction of Chlorella vulgaris Beij by Visible Spectrophotometer
中文摘要	[目的]为实现小球藻大面积推广生产和产业化发展提供科学、翔实的技术参数。[方法]通过721型分光光度计,对不同浓度的小球藻藻液在不同波段光照下的透光度进行测定,确定测定小球藻的最佳照射波段,得到最佳照射波段时小球藻藻液的浓度。[结果]在不同光照对不同浓度小球藻藻液透射的吸光度进行归一化处理后,得出小球藻的最佳透射波段为670 nm。同时,得出670 nm光照下吸光度与小球藻浓度的回归方程为:y=-1.479 7+0.987x,相关系数为0.991,说明相关性较好。推导了当量浓度(C)与透光度(I)的公式,C=-ln(I/I0)/(LK),式中L为光径长度(比色皿宽度),K为小球藻细胞的平均截面面积。[结论]用分光光度计间接测量小球藻的浓度是可行的。; [Objective]The research aimed to provide the scientific and detailed technical parameters for the achievement of massive popularization and industrialization development of Chlorella vulgaris Beij. [Method]Different concentrations of Chlorella vulgaris Beij were tested to get the light absorbance of algae by 721 Visible Spectrophotometer under 41 groups of wave bands. So the optimal wave band used to produce Chlorella could be checked out. [Result] The optimal wave band was 670 nm. This was due to the transmitted light intensity of algae got to the peak under 670 nm of incident light. The relationship between the light absorbance and the concentration of algae was y =- 1. 479 7 +0. 987 x. The relativity between them equaled 0. 991. It showed that they had good relativity. The formulate of the algae concentration( C) and the transmitted light intensity( I) was C =- ln( I /I0) /( LK). L was the width of the vessel,and K was the section area of an algae cell.[Conclusion]The indirect measurement of Chlorella vulgaris Beij by Visible Spectrophotometer was feasible.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://ir.lib.tsinghua.edu.cn/ir/item.do?handle=123456789/143697]
专题	清华大学
推荐引用方式 GB/T 7714	潘竞洋,赵之旭,吴良柏,等. 用分光光度计预测小球藻藻液浓度的研究[J],2016, 2016.
APA	潘竞洋.,赵之旭.,吴良柏.,张伟.,徐俊生.,...&Forestry Administration.(2016).用分光光度计预测小球藻藻液浓度的研究..
MLA	潘竞洋,et al."用分光光度计预测小球藻藻液浓度的研究".(2016).

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