| Bowl-shaped oligomeric structures on membranes as DegP's new functional forms in protein quality control | |
| Shen, Qing-Tao ; Bai, Xiao-Chen ; Chang, Lei-Fu ; Wu, Yi ; Wang, Hong-Wei ; Sui, Sen-Fang | |
| 2010-10-12 ; 2010-10-12 | |
| 关键词 | chaperone protease HtrA cryo-electron microscopy HEAT-SHOCK PROTEASE ESCHERICHIA-COLI CHAPERONE FUNCTION HTRA PROTEASE ACTIVATION DEGRADATION TEMPERATURE SUPPRESSES ENVELOPE STRESS Multidisciplinary Sciences |
| 中文摘要 | In the periplasm of Escherichia coli, DegP (also known as HtrA), which has both chaperone-like and proteolytic activities, prevents the accumulation of toxic misfolded and unfolded polypeptides. In solution, upon binding to denatured proteins, DegP forms large cage-like structures. Here, we show that DegP forms a range of bowl-shaped structures, independent of substrate proteins, each with a 4-, 5-, or 6-fold symmetry and all with a DegP trimer as the structural unit, on lipid membranes. These membrane-bound DegP assemblies have the capacity to recruit and process substrates in the bowl chamber, and they exhibit higher proteolytic and lower chaperone-like activities than DegP in solution. Our findings imply that DegP might regulate its dual roles during protein quality control, depending on its assembly state in the narrow bacterial envelope. |
| 语种 | 英语 ; 英语 |
| 出版者 | NATL ACAD SCIENCES ; WASHINGTON ; 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/81872]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | Shen, Qing-Tao,Bai, Xiao-Chen,Chang, Lei-Fu,et al. Bowl-shaped oligomeric structures on membranes as DegP's new functional forms in protein quality control[J],2010, 2010. |
| APA | Shen, Qing-Tao,Bai, Xiao-Chen,Chang, Lei-Fu,Wu, Yi,Wang, Hong-Wei,&Sui, Sen-Fang.(2010).Bowl-shaped oligomeric structures on membranes as DegP's new functional forms in protein quality control.. |
| MLA | Shen, Qing-Tao,et al."Bowl-shaped oligomeric structures on membranes as DegP's new functional forms in protein quality control".(2010). |
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