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	Enhanced production of medium-chain-length polyhydroxyalkanoates (PHA) by PHA depolymerase knockout mutant of Pseudomonas putida KT2442
	Cai, Lei ; Yuan, Mei-Qing ; Liu, Feng ; Jian, Jia ; Chen, Guo-Qiang
	2010-10-12 ; 2010-10-12
关键词	PHB Polyhydroxyalkanoates Pseudomonas putida Polyhydroxyalkanoates depolymerase phaZ BROAD-HOST-RANGE RALSTONIA-EUTROPHA H16 INTRACELLULAR DEPOLYMERASE ACTIVATED-SLUDGE CLONING VECTORS GENE DEGRADATION OLEOVORANS SYSTEM BIOSYNTHESIS Agricultural Engineering Biotechnology & Applied Microbiology Energy & Fuels
中文摘要	Pseudomonas putida KT2442 is a medium-chain-length polyhydroxyalkanoates (PHA) producer. One of the main shortages in the production Of PHA has been the intracellular PHA degradation caused by its endogenous PHA depolymerase. The aim of this study was to improve PHA production via removing the PHA degradation mechanism. PHA depolymerase phaZ knockout mutant A putida KTMQ01 was successfully constructed, which accumulated 86 wt% medium-chain-length PHA (mcl PHA) when cultured in mineral medium containing sodium octanoate as the carbon source compared with P. putida KT2442 which produced only 66 wt% of its cell dry weight (CDW). P. putida KTMQ01 cultured over a five-day period on sodium octanoate produced 4.5 g L (1) -4.0 g L (1) CDW containing approximately 80 wt% PHA without degradation. In contrast, A putida KT2442 was observed with decreasing CDW and PHA from over 4 to less than 2 g L-1 over the same period of time. indicating the function of PHA depolymerases which reduced the amount of PHA from around 50 wt% to none over the incubation period. RT-PCR analysis showed that phaC2 transcriptional level of A putida KTMQ01 was higher than that of P. putida KT2442, indicating the possibility of relief on negative control of phaC2 transcription by the deletion of phaZ, which combined with the lack of in vivo PHA degradation, led to more PHA accumulation. A putida KTMQ01 contained PHA granules with larger sizes and smaller numbers than those of A putida KT2442. (C) 2008 Elsevier Ltd. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCI LTD ; OXFORD ; THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81863]
专题	清华大学
推荐引用方式 GB/T 7714	Cai, Lei,Yuan, Mei-Qing,Liu, Feng,et al. Enhanced production of medium-chain-length polyhydroxyalkanoates (PHA) by PHA depolymerase knockout mutant of Pseudomonas putida KT2442[J],2010, 2010.
APA	Cai, Lei,Yuan, Mei-Qing,Liu, Feng,Jian, Jia,&Chen, Guo-Qiang.(2010).Enhanced production of medium-chain-length polyhydroxyalkanoates (PHA) by PHA depolymerase knockout mutant of Pseudomonas putida KT2442..
MLA	Cai, Lei,et al."Enhanced production of medium-chain-length polyhydroxyalkanoates (PHA) by PHA depolymerase knockout mutant of Pseudomonas putida KT2442".(2010).

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