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	Purification, crystallization and preliminary crystallographic analysis of avian infectious bronchitis virus nsp3 ADRP domain
	Lei Wei ; Cheng Chen ; Qi Zhao ; Chun Li ; Le Cong ; Xiaoling Xu ; Yanlin Ma ; Ming Liao ; Yuanyuan Xu ; Zihe Rao
	2010-10-12 ; 2010-10-12
关键词	Experimental/ biochemistry catalysis crystal purification crystallisation genetics genomics lattice constants microorganisms molecular biophysics proteins space groups/ purification crystallization crystallographic analysis avian infectious bronchitis virus nsp3 ADRP domain nonstructural proteins RNA transcription genome replication adenosine diphosphate-ribose-1'-phosphatase ADP-ribose-1'-monophosphatase catalysis tRNA-splicing pathway gene segment encoding cloning Escherichia coli gene expression crystal diffraction space group unit-cell parameters asymmetric unit/ A8715D Physical chemistry of biomolecular solutions condensed states A8725F Physics of subcellular structures A8265J Heterogeneous catalysis at surfaces and other surface reactions A8715B Biomolecular structure, configuration, conformation, and active sites
中文摘要	Avian infectious bronchitis virus (IBV) encodes 15 nonstructural proteins (nsps) which play crucial roles in RNA transcription and genome replication. One of them, nsp3, contains an ADRP (adenosine diphosphate-ribose-1'-phosphatase) domain which was revealed in recent studies to have ADP-ribose-1'-monophos- phatase (Appr-1'-pase) activity. Appr-1'-pase catalyzes the conversion of ADP- ribose-1'-monophosphate (Appr-1'-p) to ADP-ribose in the tRNA-splicing pathway. The gene segment encoding the IBV nsp3 ADRP domain has been cloned and expressed in /i Escherichia//i coli/. The protein has been crystallized and the crystals diffracted to 1.8 A resolution. They belonged to space group P1, with unit-cell parameters a = 41.1, b = 43.2, c = 48.9 A , a = 78.0, alpha = 80.0, beta = 73.6 degrees . Each asymmetric unit contains two molecules.
语种	英语
出版者	Published for the International Union of Crystallography by Munksgaard International Booksellers and Publishers Ltd. ; Denmark
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81772]
专题	清华大学
推荐引用方式 GB/T 7714	Lei Wei,Cheng Chen,Qi Zhao,et al. Purification, crystallization and preliminary crystallographic analysis of avian infectious bronchitis virus nsp3 ADRP domain[J],2010, 2010.
APA	Lei Wei.,Cheng Chen.,Qi Zhao.,Chun Li.,Le Cong.,...&Zihe Rao.(2010).Purification, crystallization and preliminary crystallographic analysis of avian infectious bronchitis virus nsp3 ADRP domain..
MLA	Lei Wei,et al."Purification, crystallization and preliminary crystallographic analysis of avian infectious bronchitis virus nsp3 ADRP domain".(2010).

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