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	Microfluidic formation of single cell array for parallel analysis of Ca2+ release-activated Ca2+ (CRAC) channel activation and inhibition
	Xu, Tao ; Li, Cheuk-Wing ; Yao, Xinsheng ; Cai, Guoping ; Yang, Mengsu
	2010-10-12 ; 2010-10-12
关键词	Microfluidics Single cell array Parallel analysis CRAC channel CULTURE ARRAY T-LYMPHOCYTES DEVICE EXPRESSION ASSAYS Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Analytical
中文摘要	High-throughput single cell analysis is required for understanding and predicting the complex stochastic responses of individual cells in changing environments. We have designed a microfluidic device consisting of parallel, independent channels with cell-docking structures for the formation of an array of individual cells. The microfluidic cell array was used to quantify single cell responses and the distribution of response patterns of calcium channels among a population of individual cells. In this device, 15 cell-docking units in each channel were fabricated with each unit containing 5 sandbag structures, such that an array of individual cells was formed in 8 independent channels. Single cell responses to different treatments in different channels were monitored in parallel to study the effects of the specific activator and inhibitor of the Ca2+ release-activated Ca2+ (CRAC) channels. Multichannel detection was performed to obtain the response patterns of the population of cells within this single cell array. The results demonstrate that it is possible to acquire single cell features in multichannels simultaneously with passive structural control, which provides an opportunity for high-throughput single cell response analysis in a microfluidic chip. (C) 2009 Elsevier Inc. All rights reserved.
语种	英语 ; 英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE ; SAN DIEGO ; 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81715]
专题	清华大学
推荐引用方式 GB/T 7714	Xu, Tao,Li, Cheuk-Wing,Yao, Xinsheng,et al. Microfluidic formation of single cell array for parallel analysis of Ca2+ release-activated Ca2+ (CRAC) channel activation and inhibition[J],2010, 2010.
APA	Xu, Tao,Li, Cheuk-Wing,Yao, Xinsheng,Cai, Guoping,&Yang, Mengsu.(2010).Microfluidic formation of single cell array for parallel analysis of Ca2+ release-activated Ca2+ (CRAC) channel activation and inhibition..
MLA	Xu, Tao,et al."Microfluidic formation of single cell array for parallel analysis of Ca2+ release-activated Ca2+ (CRAC) channel activation and inhibition".(2010).

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