Miniaturized high throughput detection system for capillary array electrophoresis on chip with integrated light emitting diode array as addressed ring-shaped light source | |
Ren, Kangning ; Liang, Qionglin ; Mu, Xuan ; Luo, Guoan ; Wang, Yiming | |
2010-10-12 ; 2010-10-12 | |
关键词 | FLUORESCENCE DETECTION SAMPLE INTRODUCTION HIGH-SPEED MICROCHIP DEVICE Biochemical Research Methods Chemistry, Multidisciplinary Nanoscience & Nanotechnology |
中文摘要 | A novel miniaturized, portable fluorescence detection system for capillary array electrophoresis (CAE) on a microfluidic chip was developed, consisting of a scanning light-emitting diode (LED) light source and a single point photoelectric sensor. Without charge coupled detector (CCD), lens, fibers and moving parts, the system was extremely simplified. Pulsed driving of the LED significantly increased the sensitivity, and greatly reduced the power consumption and photobleaching effect. The highly integrated system was robust and easy to use. All the advantages realized the concept of a portable micro-total analysis system (m-TAS), which could work on a single universal serial bus (USB) port. Compared with traditional CAE detecting systems, the current system could scan the radial capillary array with high scanning rate. An 8-channel CAE of fluorescein isothiocyanate (FITC) labeled arginine (Arg) on chip was demonstrated with this system, resulting in a limit of detection (LOD) of 640 amol. |
语种 | 英语 ; 英语 |
出版者 | ROYAL SOC CHEMISTRY ; CAMBRIDGE ; THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND |
内容类型 | 期刊论文 |
源URL | [http://hdl.handle.net/123456789/80954] |
专题 | 清华大学 |
推荐引用方式 GB/T 7714 | Ren, Kangning,Liang, Qionglin,Mu, Xuan,et al. Miniaturized high throughput detection system for capillary array electrophoresis on chip with integrated light emitting diode array as addressed ring-shaped light source[J],2010, 2010. |
APA | Ren, Kangning,Liang, Qionglin,Mu, Xuan,Luo, Guoan,&Wang, Yiming.(2010).Miniaturized high throughput detection system for capillary array electrophoresis on chip with integrated light emitting diode array as addressed ring-shaped light source.. |
MLA | Ren, Kangning,et al."Miniaturized high throughput detection system for capillary array electrophoresis on chip with integrated light emitting diode array as addressed ring-shaped light source".(2010). |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论