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	Portable optical immunosensor for highly sensitive detection of microcystin-LR in water samples
	Long, F. ; He, M. ; Zhu, A. N. ; Shi, H. C.
	2010-10-12 ; 2010-10-12
关键词	Evanescent wave immunosensor Microcystin-LR Total internal reflection Fluorescence Environmental analysis SURFACE-PLASMON RESONANCE PHOSPHATASE INHIBITION ASSAY SELF-ASSEMBLED MONOLAYER ALL-FIBER IMMUNOSENSOR CYANOBACTERIA CHROMATOGRAPHY IMMUNOASSAY CYANOTOXINS ANTIBODIES BIOSENSORS Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology
中文摘要	Fast and sensitive detection of microcystin-LR (MC-LR) was conducted with a portable trace organic pollutant analyzer (TOPA) based on the principle of immunoassay and total internal reflection fluorescence. The reusable fiber optic probe surface was produced by covalently immobilizing a MC-LR-ovalbumin (MC-LR-OVA) conjugate onto a self-assembled thiol-silane monolayer of fiber optic probe through a heterobifunctional reagent. It has been established that the MC-LR-OVA immobilized fiber optic probe is highly resistive to non-specific binding of proteins, while bound anti-MC-LR antibody can easily be eluted from the immunosurface with high recoveries (more than 150 assay cycles) in the way of pepsin solution, without any damage to the surface-immobilized MC-LR derivatives. One assay cycle including surface regeneration was less than 20 min. For the standard curve, the limit of detection (LOD) of 0.03 mu g/L and the quantitative detection range of 0.1-10.1 mu g/L was obtained when the concentration of antibody labeled by Cy5.5 was 0.28 mu g/mL. Cross-reactivity against a few compounds structurally similar to MC-LR was little. The developed immunosensor method was applied to the monitoring of MC-LR in various types of water. The recovery of MC-LR added to water samples at different concentrations ranged from 80 to 110% with relative standard deviation (R.S.D.) values less than 5%. The immunoassay performance of the TOPA was validated with respect to conventional high-performance liquid chromatography, and the correlation between methods was in good agreement (r(2) = 0.9978). The TOPA is a portable, easy-to-use, and robust immunoassay system and commercially obtained from the company JQ-environ Co. Ltd. (China). (C) 2009 Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER ADVANCED TECHNOLOGY ; OXFORD ; OXFORD FULFILLMENT CENTRE THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/79717]
专题	清华大学
推荐引用方式 GB/T 7714	Long, F.,He, M.,Zhu, A. N.,et al. Portable optical immunosensor for highly sensitive detection of microcystin-LR in water samples[J],2010, 2010.
APA	Long, F.,He, M.,Zhu, A. N.,&Shi, H. C..(2010).Portable optical immunosensor for highly sensitive detection of microcystin-LR in water samples..
MLA	Long, F.,et al."Portable optical immunosensor for highly sensitive detection of microcystin-LR in water samples".(2010).

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