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	Human umbilical cord mesenchymal stem cells: Osteogenesis in vivo as seed cells for bone tissue engineering
	Diao, Yinze ; Ma, Qingjun ; Cui, Fuzhai ; Zhong, Yanfeng
	2010-10-12 ; 2010-10-12 ; OCT
关键词	mesenchymal stem cells bone tissue engineering umbilical cord heterotopic osteogenesis in vivo MARROW STROMAL CELLS COLLAGEN MATRIX TRANSPLANTATION DIFFERENTIATION AGE PROLIFERATION ANTIBODIES REPAIR DEFECT UTERO Engineering, Biomedical Materials Science, Biomaterials
中文摘要	Mesenchymal stem cells (MSCs) are ideal seed cells for bone tissue engineering. However, intrinsic deficiencies exist for the autologous transplantation strategy of constructing artificial bone with MSCs derived from bone marrow of patients. In this study, MSCs-like cells were isolated from human umbilical cords and were expanded in vitro. Flow cytometric analysis revealed that cells from the fourth passage were positive for CD29, CD44, CD71, CD73, CD90, and CD105 whereas they were negative for CD14, CD34, CD45, and CD117. Furthermore, these cells expressed HLA-A, B, C (MHC-I), but not HLA-DP, DQ, DR (MHC-II), or costimulatory molecules such as CD80 and CD86. Following incubation in specific inductive media for 3 weeks, cultured cells were shown to possess potential to differentiate into adipogenic, osteogenic or chondrogenic lineages in vitro. The umbilical cord-derived MSCs (UC-MSCs) were loaded with a biomimetic artificial bone scaffold material before being implanted subcutaneously in the back of Balb/c nude mice for four to twelve weeks. Our results revealed that UC-MSCs loaded with the scaffold displayed capacity of osteogenic differentiation leading to osteogenesis with human origin in vivo. As a readily available source of seed cells for bone tissue engineering, UC-MSCs should have broad application prospects. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 91A: 123-131, 2009
语种	英语 ; 英语
出版者	WILEY-LISS ; HOBOKEN ; DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/78290]
专题	清华大学
推荐引用方式 GB/T 7714	Diao, Yinze,Ma, Qingjun,Cui, Fuzhai,et al. Human umbilical cord mesenchymal stem cells: Osteogenesis in vivo as seed cells for bone tissue engineering[J],2010, 2010, OCT.
APA	Diao, Yinze,Ma, Qingjun,Cui, Fuzhai,&Zhong, Yanfeng.(2010).Human umbilical cord mesenchymal stem cells: Osteogenesis in vivo as seed cells for bone tissue engineering..
MLA	Diao, Yinze,et al."Human umbilical cord mesenchymal stem cells: Osteogenesis in vivo as seed cells for bone tissue engineering".(2010).

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