| TOCSY-HSQC和NOESY-HSQC三维降二维GFT-NMR方法 | |
| 周相芝 ; 杨海军 ; 胡晓钧 ; 李勇 ; Xiangzhi Zhou ; Haijun Yang ; Xiaojun Hu ; Yong Li | |
| 2010-07-15 ; 2010-07-15 | |
| 会议名称 | 第十四届全国波谱学学术会议论文摘要集 ; Proceedings of the 14th National Magnetic Resonance Conference ; 第十四届全国波谱学学术会议 ; The 14th National Magnetic Resonance Conference ; 中国陕西西安 ; CNKI ; 中国物理学会波谱专业委员会 |
| 关键词 | Q51-3 |
| 其他题名 | Study of(3,2)D TOCSY-HSQC and(3,2)D NOESY-HSQC |
| 中文摘要 | <正>GFT-NMR 方法自出现以来,大大缩短了蛋白质化学位移归属和结构解析的时间。本文验证了 GFT-NMR 概念下的 TOCSY-HSOC 和 NOESY-HSQC 的三维降二维方法,并且利用 JEOL 的 ECA-600谱仪进行了标准样品的测试。在三维实验中,对三维都进行正交检; GFT-NMR has greatly reduced the time of chemical shifts assignment and structure analysis of proteins.In this work,we verified(3,2)D TOCSY-HSQC and(3,2)D NOESY-HSQC under the conception of GFT-NMR,and also test the pulse sequences on standard sample using JEOL ECA-600 spectrometer.In 3D experiments,all 3 Dimensions have quadrature detection in order to differ positive and negative frequencies;in(3,2)D experiments,for the simultaneously evolved ~(13)C and ~1H dimension,two sets of data were recorded: the first set of data has quadrature detection for ~(13)C and the detective pulse phase of ~1H is 0�the second set of data has quadrature detection for ~(13)C also and the detective pulse phase of ~1H is 90�.Fig.2 displays the spectrum of Ethyl Benzene(EB)from the first data set of(3,2)D NOESY-HSQC experiment.From the results,we can see the(3,2)D TOCSY-HSQC can give all the chemical shifts and cross peak information as that got from 3D TOCSY-HSQC;(3,2)D NOESY-HSQC experiment can give the chemical shifts of diagonal peaks,but gives obscure cross peaks which are hardly to observe.The relation between the intensities of cross peaks and related ~1H distances is still under studying.; 国家自然科学基金(20475032) |
| 语种 | 中文 ; 中文 |
| 内容类型 | 会议论文 |
| 源URL | [http://hdl.handle.net/123456789/68619]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 周相芝,杨海军,胡晓钧,等. TOCSY-HSQC和NOESY-HSQC三维降二维GFT-NMR方法[C]. 见:第十四届全国波谱学学术会议论文摘要集, Proceedings of the 14th National Magnetic Resonance Conference, 第十四届全国波谱学学术会议, The 14th National Magnetic Resonance Conference, 中国陕西西安, CNKI, 中国物理学会波谱专业委员会. |
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