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	SRAP-cDNA方法在植物基因差异表达分析中的应用
	桂琴 ; 王嘉璐 ; 伍晓明 ; 王建波 ; GUI Qin ; WANG Jia-lu ; WU Xiao-ming ; WANG Jian-bo
	2010-06-07 ; 2010-06-07
关键词	SRAP-cDNA 分子标记 基因差异表达 SRAP-cDNA Molecular marker Differential gene expression S188
其他题名	Application of SRAP-cDNA method in analysis of differential gene expression in plants
中文摘要	对SRAP技术的原理方法、优点进行了介绍,并阐述了SRAP-cDNA方法的实验条件优化、应用现状及前景,表明SRAP-cDNA方法具有简便、重复性好、实验成本低等优点,可以在研究植物基因差异表达领域广泛应用,并成为作物育种、杂种优势分析等工作的重要工具。; Identification of differential expressed gene is a hot spot in study of functional genomics.Several methods for identifying differences in gene expression were developed,shch as SSH,RAD,SAGE,DDRT-PCR,AFLP-cDNA,cDNA-microarray etc.SRAP is a newly developed molecular marker system.Generally,SRAP was used to fingerprint genomic DNA while seldom to fingerprint cDNA.The principle,advantage,and applications of SRAP-cDNA method were introduced in this article.The results indicated that SRAP-cDNA method had several advantages over other systems:simplicity,reasonable throughput rate,highly reproducibility and inexpensiveness.It could be widely used in analysis of differential gene expression in plants,and should be a powerful tool for study of crop breeding and heterosis.; 国家自然科学基金(30570112)
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/45721]
专题	清华大学
推荐引用方式 GB/T 7714	桂琴,王嘉璐,伍晓明,等. SRAP-cDNA方法在植物基因差异表达分析中的应用[J],2010, 2010.
APA	桂琴.,王嘉璐.,伍晓明.,王建波.,GUI Qin.,...&WANG Jian-bo.(2010).SRAP-cDNA方法在植物基因差异表达分析中的应用..
MLA	桂琴,et al."SRAP-cDNA方法在植物基因差异表达分析中的应用".(2010).

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