| 微生物发酵制备生物柴油油脂原料工艺条件的研究 | |
| 墨玉欣 ; 刘宏娟 ; 张建安 ; 程可可 ; 周玉杰 ; 陈晓辉 ; MO Yu-xin ; LIU Hong-juan ; ZHANG Jian-an ; CHENG Ke-ke ; ZHOU Yu-jie ; CHEN Xiao-hui | |
| 2010-06-04 ; 2010-06-04 | |
| 关键词 | 微生物 发酵 油脂 生物柴油 microorganism fermentation lipid biodiesel TE667 |
| 其他题名 | Study on operation conditions in production of lipid by fermentation as raw material for biodiesel |
| 中文摘要 | 以一株酵母菌为产油菌株,研究了在1 L发酵罐中发酵油脂的工艺条件,以苏丹黑染色法定量分析油脂,并对发酵油脂组成进行了气相色谱-质谱联用分析。结果表明:发酵罐最佳培养条件为30°C,通气量1.1 vvm,pH=6,转速300 r/min。分析表明发酵油脂中含有多种不饱和脂肪酸,其中软脂酸17.80%、硬脂酸10.27%、油酸53.02%、亚油酸5.29%,这与植物油组成相似。同时以微生物发酵油脂为原料,初步研究了碱催化制备生物柴油。; A kind of microzyme was used in lipid production,and the effects of operation conditions in 1 L fermentor culture were studied.The quantitative test lipid by Sudan Black B straining and confirmed the component of lipid by gas chromatograph and mass spectrum(GC-MS) were carried out.The results showed that the optimal operation conditions in 1 L fermentor culture were as follows:at 30癈,1.1 vvm of ventilation,pH=6,and 300 r/min of stirrer speed.The analysis indicated that the components of lipid produced by fermentation were mainly made of palmitic acid,steric acid,oleic acid and linoleic acid,which were 17.80%,10.27%,53.02%,and 5.29%,respectively,and the components and their portions are very like those in vegetable oil.Furthermore the lipid produced by fermentation was used as raw material to produce biodiesel with alkali as catalyst.; “211”项目资助 |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/36143]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 墨玉欣,刘宏娟,张建安,等. 微生物发酵制备生物柴油油脂原料工艺条件的研究[J],2010, 2010. |
| APA | 墨玉欣.,刘宏娟.,张建安.,程可可.,周玉杰.,...&CHEN Xiao-hui.(2010).微生物发酵制备生物柴油油脂原料工艺条件的研究.. |
| MLA | 墨玉欣,et al."微生物发酵制备生物柴油油脂原料工艺条件的研究".(2010). |
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