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	Mass spectrometric analysis for aristolochic acid-deoxyribonucleic acid adducts
	Ji Wen-Xuan ; Yang Cheng-Dui ; Liu Mi-Xin ; Chen Yi-Pu
	2010-05-11 ; 2010-05-11
关键词	aristolochic acid deoxyribonucleic acid adducts synthesis liquid chromatography-tandem mass spectrometry Fourier transform-ion cyclotron resonance mass spectrometry LIQUID-CHROMATOGRAPHY DNA-ADDUCTS CANCER Chemistry, Analytical
中文摘要	Aristolochic acid (AA) was incubated with 2'-deoxyadenosine 5'-monophosphate (dAp) in mild acid phosphate buffer in vitro for 12 hours, using either enzymatic activation (by xanthine oxidase) or chemical activation (by zinc) to synthesize AA-DNA adducts, and the solvents were evaporated under vacuum. The AA-DNA adducts were characterized by multiple mass spectrometric techniques. Electrospray ionization/tandent mass spectrometry (ESI-MS/MS) was used for the characterization of the AA-DNA adducts. Full scan spectra were obtained lit the negative]oil mode and the quasi-molecular ion peaks of the AA-DNA adducts were m/z 621 and m/z 591 respectively. Crude extracts were analyzed by, ESI-MSn technique to investigate structural information about the fragmentation rules of AA-DNA adducts in negative mode. Using the high accuracy mass data and isotope pattern Of Super high resolution Fourier transform-ion cyclotron resonance mass spectrometry ( FT-ICRMS), the AA-DNA adducts were identified further. The results indicated that AA-DNA adducts were synthesized lit vitro This method is a powerful tool for detection and identification of AA-DNA adducts. Compared with P-32-postlahelllng analysis this method has the advantages of simple operation, rapid measurement and accurate determination.
语种	中文 ; 中文
出版者	ELSEVIER SCIENCE INC ; NEW YORK ; 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/27567]
专题	清华大学
推荐引用方式 GB/T 7714	Ji Wen-Xuan,Yang Cheng-Dui,Liu Mi-Xin,et al. Mass spectrometric analysis for aristolochic acid-deoxyribonucleic acid adducts[J],2010, 2010.
APA	Ji Wen-Xuan,Yang Cheng-Dui,Liu Mi-Xin,&Chen Yi-Pu.(2010).Mass spectrometric analysis for aristolochic acid-deoxyribonucleic acid adducts..
MLA	Ji Wen-Xuan,et al."Mass spectrometric analysis for aristolochic acid-deoxyribonucleic acid adducts".(2010).

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