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N40, a novel nonacidic matrix protein from pearl oyster nacre, facilitates nucleation of aragonite in vitro
Yan, Zhenguang ; Jing, Gu ; Gong, Ningping ; Li, Changzhong ; Zhou, Yujuan ; Xie, Liping ; Zhang, Rongqing
2010-05-11 ; 2010-05-11
关键词PINCTADA-FUCATA ORGANIC MATRIX CARBONIC-ANHYDRASE HALIOTIS-LAEVIGATA PRISMATIC LAYER SHELL FORMATION MOLLUSK SHELLS GROWTH CALCITE BIOMINERALIZATION Biochemistry & Molecular Biology Chemistry, Organic Polymer Science
中文摘要A novel nonacidic matrix protein from pearl oyster nacre has been purified by cation-exchange chromatography. It was designated N40 for the nacreous protein of approximately 40 kDa. On the basis of the extraction method (with Tris-buffered Milli-Q water) and amino acid compositions (Gly- and Ala-rich), N40 was inferred to be a conventional "insoluble matrix protein". Crystallization experiments showed that N40 could facilitate the nucleation of aragonite drastically. So far, among the macromolecules that have been purified from the shell, N40 is an exclusive protein that can nucleate aragonite by itself, without the need for adsorption to a substrate. Thus, the present study has proposed the possibility that the nonacidic shell protein (maybe a conventional "insoluble framework protein") can also directly participate in aragonite nucleation and even act as a nucleation site. It is a valuable supplement to the classic biomineralization theory, in which the soluble acidic proteins of the shell are generally believed to function as a nucleation site.
语种英语 ; 英语
出版者AMER CHEMICAL SOC ; WASHINGTON ; 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
内容类型期刊论文
源URL[http://hdl.handle.net/123456789/26946]  
专题清华大学
推荐引用方式
GB/T 7714
Yan, Zhenguang,Jing, Gu,Gong, Ningping,et al. N40, a novel nonacidic matrix protein from pearl oyster nacre, facilitates nucleation of aragonite in vitro[J],2010, 2010.
APA Yan, Zhenguang.,Jing, Gu.,Gong, Ningping.,Li, Changzhong.,Zhou, Yujuan.,...&Zhang, Rongqing.(2010).N40, a novel nonacidic matrix protein from pearl oyster nacre, facilitates nucleation of aragonite in vitro..
MLA Yan, Zhenguang,et al."N40, a novel nonacidic matrix protein from pearl oyster nacre, facilitates nucleation of aragonite in vitro".(2010).
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