| A novel carbonic anhydrase from the mantle of the pearl oyster (Pinctada fucata) | |
| Yu, ZY ; Xie, LP ; Lee, SW ; Zhang, RQ | |
| 2010-05-11 ; 2010-05-11 | |
| 关键词 | acetazolamide biomineralization carbonic anhydrase histochemistry mantle pearl oyster Pinctada fucata purification LAYER FORMATION NACREOUS LAYER EDULIS L GROWTH Biochemistry & Molecular Biology Zoology |
| 中文摘要 | A novel carbonic anhydrase (CA) has been purified from the mantle of the pearl oyster, Pinctada fucata, by ammonium sulfate precipitation and affinity chromatography. Its molecular mass was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to be approximately 38 kDa. Native-PAGE shows that the novel CA can bind a fluorescent probe, 5-dimethylamino-1-naphthalenesulfonamide (DNSA), known to specifically bind carbonic anhydrase. Compared to carbonic anhydrase I (CAI) from human erythrocytes, the novel CA migrates faster indicating that it is more acidic. The effect of an inhibitor on the enzyme activity was also examined. The CA from the mantle showed a weak resistance to acetazolamide (AZ), a specific inhibitor of CA. When DNSA was bound to CA, it caused the wavelength of emission maximum intensity to blue shift to 454 nm upon excitation at 326 nm. Histochemical data indicates that the enzyme is distributed widely throughout the mantle tissue, being concentrated at the edge of the mantle. The evidence presented indicates a function for CA in the process of pearl formation and biomineralization. (C) 2005 Elsevier Inc. All rights reserved. |
| 语种 | 英语 ; 英语 |
| 出版者 | ELSEVIER SCIENCE INC ; NEW YORK ; 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/26923]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | Yu, ZY,Xie, LP,Lee, SW,et al. A novel carbonic anhydrase from the mantle of the pearl oyster (Pinctada fucata)[J],2010, 2010. |
| APA | Yu, ZY,Xie, LP,Lee, SW,&Zhang, RQ.(2010).A novel carbonic anhydrase from the mantle of the pearl oyster (Pinctada fucata).. |
| MLA | Yu, ZY,et al."A novel carbonic anhydrase from the mantle of the pearl oyster (Pinctada fucata)".(2010). |
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