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	Characterization of calcium deposition and shell matrix protein secretion in primary mantle tissue culture from the marine pearl oyster Pinctada fucata
	Gong, Ningping ; Ma, Zhuojun ; Li, Qing ; Li, Qi ; Yan, Zhenguang ; Xie, Liping ; Zhang, Rongqing
	2010-05-11 ; 2010-05-11
关键词	primary culture biomineralization calcium deposition matrix protein Pinctada fucata HALIOTIS-VARIA-LINNAEUS IN-VITRO ALKALINE-PHOSPHATASE CARBONIC-ANHYDRASE EPITHELIAL-CELLS NACRE MOLLUSK TUBERCULATA MORPHOLOGY ABALONE Biotechnology & Applied Microbiology Marine & Freshwater Biology
中文摘要	In this study, we established and characterized a long-term primary mantle tissue culture from the marine pearl oyster Pinctada fucata for in vitro investigation of nacre biomineralization. In this culture system, the viability of mantle tissue cells lasted up to 2 months. The tissue cells were demonstrated to express nacre matrix proteins by RT-PCR, and a soluble shell matrix protein, nacrein, was detected in the culture medium by Western blot analysis. On the other hand, 15 days after initiating culture, a large amount of calcium deposits with major elements, including calcium, carbon, and oxygen, were generated in the mantle explants and cell outgrowth area. The quantity and size of calcium deposits increased with the prolonged cultivation, and their location and nanogranular structure suggested their biogenic origin. These calcium deposits specifically appeared in mantle tissue cultures, but not in heart tissue cultures. Taken together, these results demonstrate that the mantle tissue culture functions similarly to mantle cells in vivo. This study provides a reliable approach for the further investigation on nacre biomineralization at the cellular level.
语种	英语 ; 英语
出版者	SPRINGER ; NEW YORK ; 233 SPRING ST, NEW YORK, NY 10013 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26837]
专题	清华大学
推荐引用方式 GB/T 7714	Gong, Ningping,Ma, Zhuojun,Li, Qing,et al. Characterization of calcium deposition and shell matrix protein secretion in primary mantle tissue culture from the marine pearl oyster Pinctada fucata[J],2010, 2010.
APA	Gong, Ningping.,Ma, Zhuojun.,Li, Qing.,Li, Qi.,Yan, Zhenguang.,...&Zhang, Rongqing.(2010).Characterization of calcium deposition and shell matrix protein secretion in primary mantle tissue culture from the marine pearl oyster Pinctada fucata..
MLA	Gong, Ningping,et al."Characterization of calcium deposition and shell matrix protein secretion in primary mantle tissue culture from the marine pearl oyster Pinctada fucata".(2010).

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